Article Figures & Data
Figures
- Figure 1.
Correlation analysis between IC50 values of DTX and β-tubulin isoform expression in 2D- and 3-cultured triple-negative breast cancers. Columns on the left and right show the 2D- and 3D-TNBCs, respectively. The x axis shows the IC50 value of DTX. The y axis shows the expression levels of TUBB isoforms obtained from qPCR based on β-actin.
- Figure 2.
Comparison of β-tubulin isoform expression levels between 2D and 3D cultures in each of 13 triple-negative breast cancer cell lines. (A) TUBB, (B) TUBB2a, (C) TUBB3, (D) TUBB4b, and (E) TUBB6. Data represent the mean±standard deviation of the two independent experiments. Number of samples (n) as noted in the graph. p-values (p) were calculated by Student’s t-test.
- Figure 3.
Comparison of β-tubulin isoform expression levels between 2D and 3D cultures in 13 triple-negative breast cancer cell lines. (A) TUBB, (B) TUBB2a, (C) TUBB3, (D) TUBB4b, and (E) TUBB6. Data represent the mean±standard deviation of two independent experiments. p-values (p) were calculated by Student’s t-test.
- Figure 4.
Typical morphology images and cell growth of 3D-cultured 13 triple-negative breast cancer cell lines. (A) Round type by spheroid morphology classification. (B) Mass type by spheroid morphology classification. (C) Grape-like type by spheroid morphology classification. The images show spheroid of each cell line on day 1 and day 5 after the start of 3D culture, and the spheroid cell area grown during the 5 days was measured and recorded using an inverted phase-contrast microscope and analyzed using the ZEN pro analysis software. Number of samples (n), spheroid area (mm2), p-value (p) and SD (standard deviation) are described in each cell lines.
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