Figure 3. Cytotoxic effects of iron chelators and cholesterol decreasing agents on neuroblastoma cells. (A) Human neuroblastoma cells (SH-SY5Y, SK-N-AS) were cultured overnight in complete cell culture medium prior to a 2-day exposure to the iron chelator DFO. (B-C) Human neuroblastoma cells (SH-SY5Y, SK-N-AS) were cultured overnight in complete cell culture medium prior to exposure to either simvastatin or ezetimibe, cholesterol decreasing agents, in medium containing 1% FBS for 2 days. (D) Human neuroblastoma CHLA-171 cells were cultured overnight in complete cell culture medium prior to a 2-day exposure to either an iron chelator (DFO) or cholesterol decreasing agent (simvastatin, ezetimibe, or U18666A) in a medium containing 1% FBS. (E, F) Human neuroblastoma cells (SK-N-AS, CHLA-171) were cultured overnight in complete cell culture medium prior to a 2-day exposure to an iron chelator, either DFO, Dp44mT, triapine, or DpC. (A-F) In each instance after two days of exposure, cytotoxicity was ascertained using the alamarBlue assay. Results represent the means of three independent experiments with error bars corresponding to standard errors. For each plot, symbols indicate statistically significant differences between treatments at the indicated concentrations. *p<0.05, **p<0.01, ***p<0.001.