Figure 6. Protein expression of Homeobox A9 (HOXA9) and its target genes in acute myeloid leukaemia cells after DB818 treatment or HOXA9 knockdown. OCI/AML3, MV4-11, and THP-1 cells were treated with 20 μM DB818 for 48 h, 10 μM DB818 for 24 h, and 20 μM DB818 for 24 h, respectively (left panels), or transfected with HOXA9 siRNA (right panels) and analysed for the expression of HOXA9, MYB proto-oncogene, transcription factor (MYB), BCL2 apoptosis regulator (BCL2), MYC proto-oncogene, bHLH transcription factor (MYC), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control, by immunoblotting.