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Research ArticleExperimental Studies

Development of Antigen-specific Chimeric Antigen Receptor KHYG-1 Cells for Glioblastoma

CHUNG HYO KANG, YEONGRIN KIM, SO MYOUNG LEE, SANG UN CHOI and CHI HOON PARK
Anticancer Research April 2021, 41 (4) 1811-1819; DOI: https://doi.org/10.21873/anticanres.14947
CHUNG HYO KANG
1Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;
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YEONGRIN KIM
1Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;
2Medicinal Chemistry and Pharmacology, Korea University of Science and Technology, Daejeon, Republic of Korea
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SO MYOUNG LEE
1Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;
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SANG UN CHOI
1Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;
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CHI HOON PARK
1Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;
2Medicinal Chemistry and Pharmacology, Korea University of Science and Technology, Daejeon, Republic of Korea
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  • For correspondence: chpark@krict.re.kr
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Abstract

Background/Aim: Glioblastoma is the most common cancer among primary brain tumors, however, its prognosis and treatment advances are very poor. Here, we investigated whether c-Met, FOLR1, and AXL proteins are promising targets for chimeric antigen receptor (CAR) T-cell therapy, for they are known to be over-expressed in a variety of solid tumors. Materials and Methods: CAR constructs were prepared and CAR KHYG-1 cells targeting c-Met, FOLR1, or AXL were made by lentiviral transduction. The activity of CAR KHYG-1 cells against cancer cells was measured by cytokine secretion and cell lysis assays. Results: c-Met and AXL were over-expressed in most glioblastoma cell lines (11/13), but not in neuroblastoma cell lines (0/8). FOLR1 was over-expressed only in one among 16 glioblastoma cell lines. Our antigen-specific CAR KHYG-1 cells eradicated target positive glioblastoma cells selectively. Conclusion: Anti-c-Met and anti-AXL CAR NK or T cells could be effective in glioblastoma cells.

Key Words:
  • Chimeric antigen receptor
  • natural killer cell
  • KHYG-1
  • c-Met
  • AXL
  • FOLR1
  • glioblastoma
  • Received February 18, 2021.
  • Revision received March 5, 2021.
  • Accepted March 8, 2021.
  • Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 41 (4)
Anticancer Research
Vol. 41, Issue 4
April 2021
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Development of Antigen-specific Chimeric Antigen Receptor KHYG-1 Cells for Glioblastoma
CHUNG HYO KANG, YEONGRIN KIM, SO MYOUNG LEE, SANG UN CHOI, CHI HOON PARK
Anticancer Research Apr 2021, 41 (4) 1811-1819; DOI: 10.21873/anticanres.14947

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Development of Antigen-specific Chimeric Antigen Receptor KHYG-1 Cells for Glioblastoma
CHUNG HYO KANG, YEONGRIN KIM, SO MYOUNG LEE, SANG UN CHOI, CHI HOON PARK
Anticancer Research Apr 2021, 41 (4) 1811-1819; DOI: 10.21873/anticanres.14947
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Keywords

  • Chimeric antigen receptor
  • natural killer cell
  • KHYG-1
  • c-Met
  • AXL
  • FOLR1
  • glioblastoma
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