Therapy-related Myeloid Leukemia With the Translocation t(8;19)(p11;q13) Leading to a KAT6A-LEUTX Fusion Gene

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Figure 1.
Examination by G-banding analysis, reverse transcription polymerase chain reaction (RT-PCR), and Sanger sequencing of the therapy-related acute myeloid leukemia (AML). A) Karyogram showing the der(8)t(8;19)(p11.2;q13.3) and der(19)t(8;19)(p11.2;q13.3). Breakpoints are indicated by arrows. B) Gel electrophoresis showing the amplified KAT6A-LEUTX cDNA fragments using the outer primer combination KAT6A-3558F1/LEUTX-320R1 (lane 1) and the amplified control ABL1 cDNA fragment using the ABL1-91F1/ABL1-404R1 primer combination (lane 2). C) Gel electrophoresis showing the amplified KAT6A-LEUTX cDNA fragments with nested PCR using the primer combinations KAT6A-3582F1/LEUTX-301R1 (lane 1) and KAT6A-3582F1/LEUTX-157R1 (lane 2). M: GeneRuler 1 Kb Plus DNA ladder (ThermoFisher Scientific). D) Partial sequence chromatograms of the cDNA amplified fragment showing the junction between exons 16 of KAT6A and 2 of LEUTX.
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Figure 2.
The proteins histone lysine acetyltransferase 6A (KAT6A) isoform 1(accession number NP_006757.2) and leucine twenty homeobox (LEUTX) isoform 1 (accession number NP_001369274.1). All known regions and sites are shown. Vertical dotted lines show the breakpoints in the various translocations.