Article Figures & Data
Figures
- Figure 1.
Growth characteristics and marker expression. A and B) Morphology of Capan-26 cells growing in a monolayer (2D) and in aggregates/spheres on an adhesive and a nonadhesive surface (3D), respectively. Scale bar – 50 μm. C) Growth curve of Capan-26 cells. n=3. D and E) Expression of CEACAM6 and CA19-9 in cells that grow in 2D and 3D systems, respectively. F) E-cadherin and cytokeratin 5/6/18 expression in Capan-26 cells.
- Figure 2.
Colony forming efficiency and stemness. A) Different types of colonies formed by Capan-26 cells in soft agar. Scale bar – 50 μm. B) Colony forming efficiency of early (20th) and late (40th) passage. C and D) Stem cell marker expression was assessed by immunofluorescence (C) or qPCR (D). n=3. In D, higher than twofold expression change was considered statistically significant.
- Figure 3.
Genetic alterations in Capan-26 cells. A) A schematic representation of KRAS deletion in Capan-26 cells. A translated truncated peptide is also depicted. B) TP53 point mutation in Capan-26 cells; Miapaca-2 cells are used as a positive control. C) Karyotyping of Capan-26 cells. Metaphase plates of aneuploid and polyploid cells are shown. Arrows depict translocation (dashed) and marker chromosomes (solid). Scale bar – 2 μm.
- Figure 4.
Sensitivity of Capan-26 cells to drug treatment. A) Capan-26 cell viability after drug treatment, determined by MTT assay. B) Cell death assessment after drug treatment. C) Proportion of cells in S phase (Click-iT Edu staining) after drug treatment. Ki-67 staining shows cells in cell cycle. Scale bar – 20 μm. D and E) Quantification of number of cells in the S phase and average Click-iT Edu fluorescence intensity, respectively. n=3.
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