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Research ArticleExperimental Studies

Amentoflavone Induces Cell-cycle Arrest, Apoptosis, and Invasion Inhibition in Non-small Cell Lung Cancer Cells

WEI-TING CHEN, CHENG-HSIEN CHEN, HUNG-TAI SU, PO-FU YUEH, FEI-TING HSU and I-TSANG CHIANG
Anticancer Research March 2021, 41 (3) 1357-1364; DOI: https://doi.org/10.21873/anticanres.14893
WEI-TING CHEN
1Department of Psychiatry, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan, R.O.C.;
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CHENG-HSIEN CHEN
2Surgical department of Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C.;
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HUNG-TAI SU
3Division of Anesthesiology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan, R.O.C.;
4School of Medicine, Tzu Chi University, Hualien, Taiwan, R.O.C.;
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PO-FU YUEH
5Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C.;
6Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.;
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FEI-TING HSU
6Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.;
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I-TSANG CHIANG
7Department of Radiation Oncology, Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C.;
8Department of Radiation Oncology, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C.;
9Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C.
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  • For correspondence: john740604@yahoo.com.tw
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Abstract

Background/Aim: Amentoflavone, an effective compound derived from medicinal plants, has been shown to boost therapeutic efficacy of chemotherapy in non-small cell lung cancer (NSCLC). However, anti-NSCLC effect of amentoflavone is ambiguous. The major purpose of the present study was to verify the inhibitory effects of amentoflavone in NSCLC cells. Materials and Methods: The effects of amentoflavone on growth and invasion of NSCLC CL-1-5-F4 cells were evaluated by cell viability assay, flow cytometry, colony formation assay, nuclear factor-kappa B (NF-κB) reporter gene assay, immunofluorescence staining, transwell invasion, and western blot assay. Results: Amentoflavone effectively induced cell growth inhibition, G1 cell-cycle arrest, apoptosis, and suppression of invasion. Furthermore, amentoflavone not only triggered expression of p27, cleaved caspase-3, -8 also reduced NF-κB signaling, protein levels of matrix metalloproteinase (MMP)-2, -9, Cyclin-D1, and vascular endothelial growth factor (VEGF).

Conclusion: Cell-cycle arrest, apoptosis induction, NF-κB signaling inhibition are associated with amentoflavone-inhibited growth and invasion of NSCLC cells.

Key Words:
  • Amentoflavone
  • non-small cell lung cancer
  • nuclear factor-kappa B
  • apoptosis
  • invasion

Footnotes

  • ↵* These Authors contributed equally to this study.

  • Authors’ Contributions

    Data curation, WT Chen, CH Chen, HT Su, PF Yueh, FT Hsu and IT Chiang; funding acquisition, WT Chen, CH Chen and HT Su; writing – original draft, FT Hsu and IT Chiang; writing – review, IT Chiang. All Authors have read and agreed to the published version of the manuscript.

  • Conflicts of Interest

    The Authors declare no conflicts of interest with the contents of this article.

  • Funding

    This study was supported by a Grant from the Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan (Grant ID: KAFGH-ZY-A-109020), Show Chwan Memorial Hospital, Changhua, Taiwan (Grant ID: SRD-108007) and Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan (Grant ID: TTCRD109-14), respectively. This work was also financially supported by the “Drug Development Center, China Medical University” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan.

  • Received January 11, 2021.
  • Revision received January 21, 2021.
  • Accepted January 22, 2021.
  • Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Amentoflavone Induces Cell-cycle Arrest, Apoptosis, and Invasion Inhibition in Non-small Cell Lung Cancer Cells
WEI-TING CHEN, CHENG-HSIEN CHEN, HUNG-TAI SU, PO-FU YUEH, FEI-TING HSU, I-TSANG CHIANG
Anticancer Research Mar 2021, 41 (3) 1357-1364; DOI: 10.21873/anticanres.14893

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Amentoflavone Induces Cell-cycle Arrest, Apoptosis, and Invasion Inhibition in Non-small Cell Lung Cancer Cells
WEI-TING CHEN, CHENG-HSIEN CHEN, HUNG-TAI SU, PO-FU YUEH, FEI-TING HSU, I-TSANG CHIANG
Anticancer Research Mar 2021, 41 (3) 1357-1364; DOI: 10.21873/anticanres.14893
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Keywords

  • Amentoflavone
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  • nuclear factor-kappa B
  • apoptosis
  • invasion
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