Figure 1. The YYB-101 effect on HGF-induced motility and the synergistic effect of paclitaxel and YYB-101 on ovarian cancer cell migration. (A) A2780/luc cells (3×105) and SKOV3 cells (1×105) were loaded into the upper Transwell compartments, while the lower chambers contained 0.3 μM YYB-101, 80 ng/ml HGF, or 80 ng/ml HGF with 0.3 μM or 3 μM YYB-101. After incubation for 48 h (A2780/luc cells) or 8 h (SKOV3 cells), migrated cells were fixed, stained, and observed using light microscope (10×). (B) For invasion assay, A2780/luc cells (2×105) and SKOV3 cells (0.5×105) were loaded onto Matrigel-coated upper Transwell compartments. Lower chambers contained 0.3 μM YYB-101, 80 ng/mL HGF, or 80 ng/ml HGF with 0.3 μM or 3 μM YYB-101. After culturing for 72 h (A2780/luc cells) or 48 h (SKOV3 cells), invaded cells were fixed, stained, and observed using light microscope (10×). (C) SKOV3 (1×104) cells were seeded into 96-well culture plates. After incubation 24 h, the scratch wound was formed by a wound maker, and the cells were treated with 1 pM paclitaxel, 3 μM YYB-101, or 1 pM paclitaxel with 3 μM YYB-101, and images were taken at the indicated times in the same field of each well. (D) The relative wound density was recorded over time using the IncuCyte ZOOM live-cell imaging program.