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Research ArticleExperimental Studies

Profiling of Aldehyde Dehydrogenase Isoforms in In Vitro Formed Tumorspheres

ILIAS TSOCHANTARIDIS, GEORGIA-PERSEPHONI VOULGARIDOU, ALEXANDRA GIATROMANOLAKI, MICHAEL I. KOUKOURAKIS, MIHALIS I. PANAYIOTIDIS and AGLAIA PAPPA
Anticancer Research November 2021, 41 (11) 5481-5488; DOI: https://doi.org/10.21873/anticanres.15361
ILIAS TSOCHANTARIDIS
1Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis, Greece;
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GEORGIA-PERSEPHONI VOULGARIDOU
1Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis, Greece;
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ALEXANDRA GIATROMANOLAKI
2Department of Pathology, University General Hospital of Alexandroupolis, Democritus University of Thrace, Alexandroupolis, Greece;
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MICHAEL I. KOUKOURAKIS
3Radiotherapy/Oncology, Department of Medicine, School of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece;
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MIHALIS I. PANAYIOTIDIS
4Department of Cancer Genetics, Therapeutics & Ultrastructural Pathology, The Cyprus Institute of Neurology & Genetics, Nicosia, Cyprus;
5School of Molecular Medicine, The Cyprus Institute of Neurology & Genetics, Nicosia, Cyprus
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AGLAIA PAPPA
1Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis, Greece;
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  • For correspondence: apappa@mbg.duth.gr
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Abstract

Background/Aim: Aldehyde dehydrogenases (ALDHs) are considered as markers for normal and cancer stem cells (CSC) and are involved in cell metabolism, proliferation, differentiation, stemness, and retinoic acid (RA) biosynthesis. The aim of the present study was to identify the ALDH isoforms that are associated with the CSC phenotype in non-small cell lung and hepatocellular carcinomas. Materials and Methods: We utilized lung (A549) and hepatocellular (HepG2) cancer cells and generated tumor spheres to isolate the CSC sub-population. Results: The CSC enrichment was confirmed by the up-regulation of various CSC-related genes. Comparative qPCR analysis indicated the up-regulation of several ALDH isoforms in A549 and HepG2 spheres. Interestingly, cyclin D1 and Akt, down-stream targets of the RA signaling pathway, were also shown to be significantly up-regulated in both sphere populations. Conclusion: Specific ALDH isoforms appear to be important mediators for the acquisition of an CSC phenotype and thus, are potential promising targets for CSC-based therapeutic approaches in lung and hepatocellular carcinomas.

Key Words:
  • ALDH
  • ALDH3A1
  • cancer stem cells (CSCs)
  • CSC markers
  • A549
  • HepG2
  • Received September 24, 2021.
  • Revision received October 14, 2021.
  • Accepted October 15, 2021.
  • Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 41 (11)
Anticancer Research
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November 2021
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Profiling of Aldehyde Dehydrogenase Isoforms in In Vitro Formed Tumorspheres
ILIAS TSOCHANTARIDIS, GEORGIA-PERSEPHONI VOULGARIDOU, ALEXANDRA GIATROMANOLAKI, MICHAEL I. KOUKOURAKIS, MIHALIS I. PANAYIOTIDIS, AGLAIA PAPPA
Anticancer Research Nov 2021, 41 (11) 5481-5488; DOI: 10.21873/anticanres.15361

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Profiling of Aldehyde Dehydrogenase Isoforms in In Vitro Formed Tumorspheres
ILIAS TSOCHANTARIDIS, GEORGIA-PERSEPHONI VOULGARIDOU, ALEXANDRA GIATROMANOLAKI, MICHAEL I. KOUKOURAKIS, MIHALIS I. PANAYIOTIDIS, AGLAIA PAPPA
Anticancer Research Nov 2021, 41 (11) 5481-5488; DOI: 10.21873/anticanres.15361
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Keywords

  • ALDH
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  • cancer stem cells (CSCs)
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  • A549
  • HepG2
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