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Research ArticleExperimental Studies

Effects of Hypoxia on Proliferation and Apoptosis of Osteosarcoma Cells

SHANG-YOU YANG, ELKA GARCIA, WILL XIA and ANYU WANG
Anticancer Research October 2021, 41 (10) 4781-4787; DOI: https://doi.org/10.21873/anticanres.15293
SHANG-YOU YANG
1Department of Orthopaedic Surgery, University of Kansas School of Medicine, Wichita, KS, U.S.A.;
2Department of Biological Sciences, Wichita State University, Wichita, KS, U.S.A.;
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  • For correspondence: syang6@kumc.edu anyuw@tom.com
ELKA GARCIA
2Department of Biological Sciences, Wichita State University, Wichita, KS, U.S.A.;
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WILL XIA
2Department of Biological Sciences, Wichita State University, Wichita, KS, U.S.A.;
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ANYU WANG
1Department of Orthopaedic Surgery, University of Kansas School of Medicine, Wichita, KS, U.S.A.;
3Department of Endocrinology, Zunyi Municipal Hospital of Traditional Chinese Medicine, Zunyi, Guizhou, P.R. China
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  • For correspondence: syang6@kumc.edu anyuw@tom.com
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Abstract

Background: Hypoxia can happen during solid tumor growth including osteosarcoma. This study investigated the relationship of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) on osteosarcoma cell growth and apoptosis under hypoxic conditions. Materials and Methods: Human osteosarcoma cells were cultured under normal or hypoxic conditions. Inhibitors of HIF-1α and VEGF were applied to the cells separately or in combination to block the respective proteins. Cell proliferation and apoptosis were examined by MTT and TUNEL assays, and real-time PCR and ELISA were performed for mRNA and protein expression. Results: There was a dramatic decrease of cell proliferation and an elevation of apoptosis under hypoxia. Blockage of HIF-1α and VEGFR enhanced the cell growth retardation and promoted apoptotic changes. Moreover, blockage of HIF-1α significantly eliminated the expression of VEGF in the cell culture media, and vice versa. Conclusion: HIF-1α and VEGF work closely in regulating osteosarcoma cell growth under hypoxic conditions and blockage of either of them may subsequently influence the presence of the other.

Key Words:
  • Hypoxia
  • osteosarcoma
  • HIF-1α
  • VEGF
  • apoptosis
  • Received April 2, 2021.
  • Revision received August 25, 2021.
  • Accepted August 31, 2021.
  • Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 41 (10)
Anticancer Research
Vol. 41, Issue 10
October 2021
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Effects of Hypoxia on Proliferation and Apoptosis of Osteosarcoma Cells
SHANG-YOU YANG, ELKA GARCIA, WILL XIA, ANYU WANG
Anticancer Research Oct 2021, 41 (10) 4781-4787; DOI: 10.21873/anticanres.15293

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Effects of Hypoxia on Proliferation and Apoptosis of Osteosarcoma Cells
SHANG-YOU YANG, ELKA GARCIA, WILL XIA, ANYU WANG
Anticancer Research Oct 2021, 41 (10) 4781-4787; DOI: 10.21873/anticanres.15293
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Keywords

  • hypoxia
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  • VEGF
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