Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Anticancer Research
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Anticancer Research

Advanced Search

  • Home
  • Content
    • Current
    • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Visit us on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

When Is Immunohistochemistry Useful in Assessing Tumor Necrotic Tissue?

ELZBIETA BOGAJEWSKA-RYLKO, NAVID AHMADI, MARTA PYREK, BEATA DZIEKAN, VIOLETTA FILAS, ANDRZEJ MARSZAŁEK and LUKASZ SZYLBERG
Anticancer Research January 2021, 41 (1) 197-201; DOI: https://doi.org/10.21873/anticanres.14765
ELZBIETA BOGAJEWSKA-RYLKO
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
NAVID AHMADI
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MARTA PYREK
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
BEATA DZIEKAN
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
VIOLETTA FILAS
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
ANDRZEJ MARSZAŁEK
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
LUKASZ SZYLBERG
1Chair and Department of Oncologic Pathology and Prophylactics, Greater Poland Cancer Center, Poznań University of Medical Sciences and Department of Oncologic Pathology, Poznan, Poland;
2Department of Tumor Pathology and Pathomorphology, Oncology Center, Prof. Franciszek Łukaszczyk Memorial Hospital, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland;
3Chair of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: l.szylberg@cm.umk.pl
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Background/Aim: Immunohistochemistry (IHC) enables visualisation of the distribution of specific proteins, the differentiation of benign and malignant tumours, and the site and origin of a primary tumour. Surgical pathologists commonly examine tumours with extensive necrosis or non-viable tissue that may affect an accurate diagnosis. Materials and Methods: We investigated the sensitivity and specificity of IHC on necrotic samples derived from adenocarcinoma, squamous cell carcinoma (SCC) and melanoma using different markers. Results: Analysis of necrosis within tumours revealed 88% sensitivity and 56% specificity for melanoma, 95% and 92% for CK5/6, 95% and 83% for CK20, 37% and 95% for p63, 69% and 97% for Melan A, 88% and 92% for SOX-10, 98% and 56% for CKAE/AE3 and 75% specificity for CK7. Conclusion: Antibodies should be considered reliable markers for demonstrating the epithelial nature of a suspected tumour. Immunohistochemistry of necrotic tissues may provide clinically useful information.

Key Words:
  • Immunohistochemistry
  • diagnosis
  • necrosis
  • tumours
  • Received November 8, 2020.
  • Revision received November 24, 2020.
  • Accepted November 26, 2020.
  • Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
View Full Text

This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.

patientACCESS

patientACCESS - Patients desiring access to articles
PreviousNext
Back to top

In this issue

Anticancer Research: 41 (1)
Anticancer Research
Vol. 41, Issue 1
January 2021
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Anticancer Research.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
When Is Immunohistochemistry Useful in Assessing Tumor Necrotic Tissue?
(Your Name) has sent you a message from Anticancer Research
(Your Name) thought you would like to see the Anticancer Research web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
13 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
When Is Immunohistochemistry Useful in Assessing Tumor Necrotic Tissue?
ELZBIETA BOGAJEWSKA-RYLKO, NAVID AHMADI, MARTA PYREK, BEATA DZIEKAN, VIOLETTA FILAS, ANDRZEJ MARSZAŁEK, LUKASZ SZYLBERG
Anticancer Research Jan 2021, 41 (1) 197-201; DOI: 10.21873/anticanres.14765

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
When Is Immunohistochemistry Useful in Assessing Tumor Necrotic Tissue?
ELZBIETA BOGAJEWSKA-RYLKO, NAVID AHMADI, MARTA PYREK, BEATA DZIEKAN, VIOLETTA FILAS, ANDRZEJ MARSZAŁEK, LUKASZ SZYLBERG
Anticancer Research Jan 2021, 41 (1) 197-201; DOI: 10.21873/anticanres.14765
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • Inhibition of Invasion by Polyphenols from Citrus Fruit and Berries in Human Malignant Glioma Cells In Vitro
  • Optimization of an E1A Gene Expression Cassette in an Oncolytic Adenovirus for Efficient Tumor Cell Killing Activity
  • Reversion from Methionine Addiction to Methionine Independence Results in Loss of Tumorigenic Potential of Highly-malignant Lung-cancer Cells
Show more Experimental Studies

Similar Articles

Keywords

  • immunohistochemistry
  • diagnosis
  • necrosis
  • tumours
Anticancer Research

© 2021 Anticancer Research

Powered by HighWire