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Research ArticleExperimental Studies

MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues

ALEXANDRA KISS, VIKTÓRIA BAKSA, MIKLÓS BEGE, LÁSZLÓ TÁLAS, ANIKÓ BORBÁS, ILONA BERECZKI, GÁSPÁR BÁNFALVI and GÁBOR SZEMÁN-NAGY
Anticancer Research January 2021, 41 (1) 137-149; DOI: https://doi.org/10.21873/anticanres.14759
ALEXANDRA KISS
1Department of Molecular Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary;
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VIKTÓRIA BAKSA
1Department of Molecular Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary;
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MIKLÓS BEGE
2Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary;
3MTA-DE Molecular Recognition and Interaction Research Group, University of Debrecen, Debrecen, Hungary
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LÁSZLÓ TÁLAS
1Department of Molecular Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary;
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ANIKÓ BORBÁS
2Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary;
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ILONA BERECZKI
2Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary;
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GÁSPÁR BÁNFALVI
1Department of Molecular Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary;
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  • For correspondence: gaspar.banfalvi@gmail.com bgaspar@unideb.hu
GÁBOR SZEMÁN-NAGY
1Department of Molecular Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary;
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Abstract

Background/Aim: Conventional viability tests, help to screen the cellular effects of candidate molecules, but the endpoint of these measurements lacks sufficient information regarding the molecular aspects. A non-invasive, easy-to-setup live-cell microscopic method served to in-depth analysis of mechanisms of potential anticancer drugs. Materials and Methods: The proposed method combining the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test with time-lapse scanning microscopy (TLS), provided additional data related to the cell-cycle and the dynamic properties of cell morphology. Apoptotic and necrotic events became detectable with these methods. Results: Quantification of the results was assisted by image analysis of the acquired image sequences. After demonstrating the potential of the TLS method, a series of experiments compared the in vitro effect of a known and a newly synthesized nucleoside analogue. Conclusion: The proposed approach provided a more in-depth insight into the cellular processes that can be affected by known chemotherapeutic agents including nucleoside analogues rather than applying repeated individual treatments.

Key Words:
  • Antimetabolites
  • nucleoside analogues
  • cellular morphology
  • time-lapse microscopy
  • Received October 13, 2020.
  • Revision received November 12, 2020.
  • Accepted November 19, 2020.
  • Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 41 (1)
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MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues
ALEXANDRA KISS, VIKTÓRIA BAKSA, MIKLÓS BEGE, LÁSZLÓ TÁLAS, ANIKÓ BORBÁS, ILONA BERECZKI, GÁSPÁR BÁNFALVI, GÁBOR SZEMÁN-NAGY
Anticancer Research Jan 2021, 41 (1) 137-149; DOI: 10.21873/anticanres.14759

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MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues
ALEXANDRA KISS, VIKTÓRIA BAKSA, MIKLÓS BEGE, LÁSZLÓ TÁLAS, ANIKÓ BORBÁS, ILONA BERECZKI, GÁSPÁR BÁNFALVI, GÁBOR SZEMÁN-NAGY
Anticancer Research Jan 2021, 41 (1) 137-149; DOI: 10.21873/anticanres.14759
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Keywords

  • Antimetabolites
  • nucleoside analogues
  • cellular morphology
  • time-lapse microscopy
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