Abstract
Background/Aim: Triple negative breast cancer (TNBC) is an aggressive type of breast cancer with limited targets for chemotherapy. This study evaluated the inhibitory effects of novel imidazo[2,1-b]oxazole-based rapidly accelerated fibrosarcoma (RAF) inhibitors, KIST0215-1 and KIST0215-2, on epithelial cell transformation and TNBC tumorigenesis. Materials and Methods: Immunoblotting, BrdU incorporation assay, reporter gene assay, and soft agar assay analyses were performed. In vivo effects were studied using the BALB/c mouse xenograft model. Results: KIST0215-1 and KIST0215-2 inhibited the RAFs-MEK1/2-ERK1/2 signalling pathway induced by EGF in MDA-MB-231 cells, which inhibited c-fos transcriptional activity and activator protein-1 transactivation activity. KIST0215-1 and KIST0215-2 also prevented neoplastic transformation of JB6 C141 mouse epidermal cells induced by EGF and consistently suppressed the growth of tumours formed by 4T1 cells in BALB/c mice. Conclusion: Inhibition of RAF kinases using KIST0215-1 and KIST0215-2 is a promising chemotherapeutic strategy to treat TNBC.
Breast cancer is the leading cause of cancer-related deaths in women worldwide (1). Clinically, breast cancer is characterised based on the expression levels of the oestrogen receptor, progesterone receptor (PgR), and human epidermal growth factor-2 (HER2) receptor (2). These receptors are not expressed in triple negative breast cancer (TNBC) patients, and therefore, do not respond to hormone targeted therapies, such as tamoxifen, and to specific antibodies, such as trastuzumab, which are effective in other types of breast cancers (1, 3, 4). Currently, TNBC is treated with adjuvant chemotherapies, such as platinum compounds, taxanes, and anthracycline derivatives, combined with surgery and radiotherapy (5). However, high drug toxicity and relapse rates limit the therapeutic usefulness of these compounds (5). Numerous efforts have been made to develop targeted chemotherapies for TNBC. Several compounds with novel targets such as PARP, VEGF/VEGFR, EGFR, PI3K, HDAC or mTOR are potential chemotherapy candidates in TNBC (5-7). However, these compounds have not been as clinically successful as anticipated. Thus, alternative molecular targets have to be identified to develop truly effective targeted chemotherapies.
Several cancer driver genes are either mutated or amplified in TNBC, making it a heterogenous disease (1, 4). Mitogen-activated protein kinase (MAPK) signalling pathway up-regulation is frequently seen in TNBC (8-10). The Cancer Genome Atlas dataset analysis has revealed that approximately 80% of basal-like TNBC have some degree of genomic amplification or activation of major components of the MAPK signalling pathway (11). MAPK signalling is a cascade of protein kinases, regulating cell proliferation, survival, and apoptosis in response to growth factors such as EGF, IGF-1, or PDGF (10, 12). Activation of the RAS oncogene is the initial step in the activation of the MAPK pathway (13). Oncogenic RAS recruits RAF kinases, a family of serine/threonine-specific protein kinases composed of A-RAF, B-RAF and C-RAF, to the cell membrane and phosphorylates serine or threonine residues (13). RAF kinases then activate MEK1/2 by phosphorylating multiple serine residues. MEK1/2 subsequently phosphorylates ERK1/2, leading to their activation (13). MEK1/2 and ERK1/2 are dual-specificity kinases that modulate the expression of several cancer driver genes via the phosphorylation and activation of transcription factors such as c-Fos, c-Jun, c-Myc, and activator protein-1 (AP-1) (13). Sustained activation of the MAPK pathway leads to the enhanced proliferation of TNBC cancer cells.
Many cancer cell types harbour mutations in the RAS and RAF genes, whose proteins are constitutively active (10). Activating mutations in the RAS oncogene are most predominant in pancreatic adenocarcinoma (90%), colorectal (50%), thyroid (50%), and lung cancers (30%) (10). Activating mutations in B-RAF oncogenes are mostly found in melanoma (70%), thyroid (40-70%), and colorectal carcinomas (18%) (10). The high frequency of activating RAS and RAF oncogene mutations in cancer has led to the development of small molecule inhibitors for these proteins (10, 13, 14). However, RAS inhibitors are not feasible for various reasons including high affinity of RAS for GTP (10). RAF inhibitors including vemurafenib, sorafenib, and dabrafenib have been clinically approved for treating melanoma, non-small-cell lung cancer, renal cell carcinoma, and thyroid cancer (15).
MAPK pathway up-regulation is frequently observed in TNBC due to wild-type B-RAF and C-RAF overexpression. The Cancer Genome Atlas database analysis revealed that the B-RAF gene is amplified in approximately 31% of TNBC specimens, with activating B-RAF gene mutations in only 1-2% of cases (11). Therefore, RAF kinase inhibitors are suggested as potential candidates for the chemotherapy of TNBC patients (16). However, clinically approved RAF kinase inhibitors have failed to yield clinical benefits for TNBC patients. RAF kinase inhibitors, such as vemurafenib and dabrafenib, are effective on tumours containing the B-RAF-V600E mutation but are not indicated for TNBC patients, since the V600E mutation is usually absent in TNBC (17). Sorafenib, which inhibits multiple kinases, including C-RAF, has modest activity against TNBC when combined with capecitabine or paclitaxel (18, 19). However, sorafenib failed to demonstrate a similar clinical benefit against TNBC during a phase III clinical trial (20). Sorafenib belongs to the class of ATP-competitive inhibitors, which are relatively poor inhibitors of wild-type B-RAF kinase (21). Indeed, ATP-competitive RAF inhibitors might lead to the paradoxical activation of the MAPK pathway in B-RAF wild-type cancer cells, including TNBC, by promoting B-RAF dimerization (22). Pan-RAF inhibitors, which inhibit all three forms of RAF kinases, may avoid the paradoxical activation of MAPK pathway (23). Furthermore, pan-RAF inhibitors have an enhanced therapeutic response in mutant cancer cells lacking B-RAF-V600E, such as TNBC cells (23). Therefore, to explore the therapeutic efficacy of RAF inhibitors against TNBC, it is necessary to develop novel pan-RAF kinase inhibitors or oncogenic B-RAF and C-RAF kinase inhibitors.
Here, we developed two novel imidazo[2,1-b] oxazole derivatives, KIST0215-1 and KIST0215-2, as dual inhibitors of B-RAF and C-RAF kinases and show that inhibition of RAF kinases using these compounds reduces MDA-MB-231 cell proliferation. KIST0215-1 and KIST0215-2 reduced the MEK1/2 and ERK1/2 phosphorylation induced by EGF. Furthermore, these novel compounds also induced apoptosis and cell cycle arrest in MDA-MB-231 cells. Our study findings support the therapeutic potential of dual RAF inhibitors in TNBC treatment.
Materials and Methods
Cell lines and establishment of stable cell lines. MDA-MB-231 and 4T1 breast cancer cells were grown in Eagle's minimal essential medium and Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum, respectively. All cell lines were cultured and maintained at 37°C in a humidified atmosphere containing 5% CO2.
Reagents and antibodies. Antibodies against MEK1/2 (1:1,000), ERK1/2 (1:1,000), c-Fos (1:1,000), total caspase 3 (1:1,000), phospho-MEKs (1:1,000), phospho-ERKs (1:1,000), phospho-c-Fos (1:1,000), and cleaved caspase 3 (1:1,000) were acquired from Cell Signalling Technology Inc. (Danvers, MA, USA). The antibody against PARP (1:5,000) was acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse IgG-HRP and anti-rabbit IgG-HRP were purchased from Invitrogen (Carlsbad, CA, USA).
Mammalian expression vectors. The c-fos-luc promoter (pFos WT-GL3) was provided by Dr. Ron Prywes (Columbia University, New York, NY). The AP-1 luciferase reporter plasmid (pGL4.44[luc2P/AP1 RE/Hygro] vector; Cat. No., E4111) was purchased from Promega (Madison, WI, USA).
Immunoblot assay. The cells were disrupted in RIPA buffer containing 150 mM NaCl, 50 mM Tris-HCl (pH 7.4), 0.25% sodium deoxycholate, 1 mM EDTA, 1% NP40, 1 mM NaF, 0.2 mM phenylmethyl sulfonyl fluoride, 0.1 mM sodium orthovanadate, and a protease inhibitor cocktail (Roche Life Science, Indianapolis, IN, USA). The proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA) blocked in 5% skim milk and probed with the indicated antibodies. The immunoblots were visualised using a SuperSignal West Femto chemiluminescence substrate (Thermo Fisher Scientific, Waltham, MA, USA) and detected using the LAS 4000-mini biomolecular imaging system (FUJIFILM, Tokyo, Japan).
In vitro kinase assay. Kinase assay was performed as described previously (24). Briefly, the Reaction Biology Corp. Kinase HotSpotSM service was used for the kinase assay using 1 μM concentration of ATP.
Cell proliferation assay (BrdU incorporation). Cell proliferation was assessed using a cell proliferation ELISA kit (Roche Life Sciences, Penzberg, Germany) according to the manufacturer's instructions. Briefly, MDA-MB-231 cells were seeded (5,000 cells per well) in 96-well plates in 100 μl of medium supplemented with 10% FBS. After 24 h, the cells were treated with KIST0215-1 and KIST0215-2 for 48 h, labelled with 10 μL/well BrdU-labelling solution, and incubated for an additional 4 h at 37°C in 5% CO2. The medium containing the BrdU-labelling reagent was aspirated, and FixDenat solution was added into each well. The plate was incubated at room temperature (RT) for 30 min and the solution was removed. Anti-BrdU-POD-working solution was then added to each well and incubated for a further 90 min at RT. The cells were washed three times with washing solution, followed by addition of 100 μl of substrate solution to each well and incubation for another 30 min. Cell proliferation was estimated by measuring the absorbance at 370 nm.
Reporter gene assay. The reporter gene assay for firefly luciferase activity was performed using lysates from AP-1 or c-Fos transfected JB6 Cl41 cells. In addition, the reporter gene vector pRL-TK-luciferase plasmid (Promega) was co-transfected into each cell line, and the Renilla luciferase activity generated by this vector was used to normalise the results for transfection efficiency. Cell lysates were prepared by washing the transfected JB6 Cl41 cells once with phosphate-buffered saline (PBS). After removing the PBS completely, passive lysis buffer (Promega) was added, and the cells were incubated at RT for 1 h with gentle shaking. The supernatant fraction was used to measure firefly and Renilla luciferase activities. Cell lysates (50 μl each) were mixed with 50 μl of luciferase assay II reagent (Promega), and firefly luciferase catalysed light emission was measured using a Glomax luminometer (Promega). Subsequently, 50 μl of Renilla luciferase substrate was added, and the luminescence produced was measured.
Anchorage-independent cellular transformation assay. The effect of KIST0215-1 and KIST0215-2 on transformation was investigated in JB6 C141 cells. Briefly, 8×103 cells were exposed to the indicated doses of KIST0215-1 or KIST0215-2 in 1 ml of 0.3% Eagle's basal medium agar containing 10% FBS, 2 mM L-glutamine, and 25 μg/ml gentamicin. The cultures were maintained at 37°C in a 5% CO2 incubator for 14-20 days. Cell colonies were scored using an Axiovert 200 M fluorescence microscope and Axio Vision software (Carl Zeiss Inc., Thornwood, NY, USA).
Detection of apoptosis. The induction of apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining and detected with an in situ Cell Death detection kit (Roche Life Sciences). Briefly, 3×105 cells were cultured for 24 h in 6-well plates. The cells were then exposed to indicated concentrations of KIST0215-1 and KIST0215-2 for 24 h. The treated cells were washed with PBS and fixed with Cytofix/Cytoperm reagent (BD Biosciences, San Diego, CA, USA) at 4°C for 20 min. Cells were stained with 50 μl TUNEL solution at 37°C for 1 h, washed twice with PBS, and fixed. DNA fragmentation was detected using the Axiovert 200 M fluorescence microscope.
Tumorigenicity assay in mice. Six-week-old female BALB/c mice (18-20 g) were obtained from Samtako Co. (Osan, Republic of Korea). The mice were acclimatised for 1 week and maintained in a clean room at the College of Pharmacy, Chosun University. Animal study protocols were approved by the Animal Care Committee of Chosun University. Mice were randomly divided into two or four groups of 20 animals each, and 4T1 cells were trypsinised, washed, resuspended with PBS, and adjusted to a concentration of 1×106 cells/100 μl in PBS. Cells were injected into the mammary gland of the mice together with 50 μM KIST0215-1 and/or KIST0215-2 and allowed to form tumours. The tumour volume was calculated using the formula: tumour volume=0.5× [(large diameter) × (short diameter)2].
Cell cycle assay. MDA-MB-231 cells were seeded (3.0×105 cells per plate) in 60 mm dishes in 3 ml of Modified Eagle's medium supplemented with 10% FBS and incubated at 37°C in 5.0% CO2. After 24 h, cells were treated with 20 μM of KIST0215-1 and KIST0215-2 and incubated for 48 h. Cells were harvested and fixed with 500 μl of 70% cold ethanol at −20°C and analysed using the Muse Cell Analyser (Millipore, Billerica, MA, USA) according to the manufacturer's instructions.
Statistical analysis. Statistical calculations were carried out using Prism 4 for Macintosh (GraphPad Software Inc., La Jolla, CA, USA). Results are expressed as the mean±standard error of triplicate measurements of three independent experiments. For multiple comparison analyses, a one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test was used. Differences were considered significant when the calculated p-value was <0.05.
Results
KIST0215-1 and KIST0215-2 inhibits cell proliferation and MAPK signalling in MDA-MB-231 cells. An inhibition assay directed at B-RAF and C-RAF kinases was performed using the novel imidazo[2,1-b]oxazole compounds, KIST0215-1 and KIST0215-2, respectively (Figure 1A and B). Both compounds displayed inhibitory concentration (IC50) in nanomolar ranges against B-RAF and C-RAF kinases (Figure 1C). Therefore, KIST0215-1 and KIST0215-2 were characterised as B-RAF and C-RAF inhibitors. Next, we examined whether these compounds can reduce the MDA-MB-231 cell proliferation using the BrdU incorporation assay. They significantly reduced cell proliferation in a dose-dependent manner (Figure 2A). To investigate whether KIST0215-1 and KIST0215-2 can inhibit MEK1/2 and ERK1/2 phosphorylation, MDA-MB-231 cells were treated with various doses of KIST0215-1 and KIST0215-2 for various times. The levels of phosphorylated MEK1/2 and ERK1/2 was detected by immunoblotting. KIST0215-1 and KIST0215-2 inhibited MEK1/2, ERK1/2, and c-Fos phosphorylation in a dose- and time-dependent manner (Figure 2B and 2C). To examine the effects of KIST0215-1 and KIST0215-2 on MEK1/2 and ERK1/2 phosphorylation induced by EGF, MDA-MB-231 cells were exposed to EGF in the absence or presence of various concentrations of KIST0215-1 and KIST0215-2. Both compounds inhibited the EGF-induced phosphorylation of MEK1/2, ERK1/2, and c-Fos (Figure 2D). Hence, these data suggested that KIST0215-1 and KIST0215-2 inhibit the RAFs-MEK1/2-ERK1/2 signalling pathway, and thereby inhibit MDA-MB-231 cell proliferation.
KIST0215-1 and KIST0215-2 inhibit EGF-induced c-Fos and AP-1 promoter activity. The AP-1 transcription factor is important in TNBC tumorigenesis (25). AP-1 is a heterodimer composed of Jun and Fos proteins, whose activity is regulated by the MAPK pathway in response to growth factors including EGF. To ascertain whether KIST0215-1 and KIST0215-2 can suppress c-Fos and AP-1 transcriptional activity in MDA-MB-231 cells, reporter plasmids carrying the luciferase gene under the control of c-Fos promoter or AP-1 response elements were used. The c-Fos and AP-1 activities were inhibited by KIST0215-1 and KIST0215-2 in a dose-dependent manner (Figure 3A and B). These compounds also suppressed the EGF-induced c-Fos and AP-1 promoter activities in a dose-dependent manner (Figure 3C and D). These results indicated that KIST0215-1 and KIST0215-2 can inhibit the activities of c-Fos and AP-1 transcription factors induced by the RAF-MEK1/2-ERK1/2 pathway in MDA-MB-231 cells.
KIST0215-1 and KIST0215-2 induces apoptosis and cell cycle arrest in MDA-MB-231 cells. Next, we examined whether KIST0215-1 and KIST0215-2 can regulate apoptosis. We determined the levels of cleaved caspase 3 and cleaved-PARP using immunoblotting. KIST0215-1 and KIST0215-2 induced the cleavage of caspase 3 and PARP in a dose-dependent manner (Figure 4A). Furthermore, these compounds induced cell cycle arrest at the G0/G1 phase (Figure 4B). Consistent with previous results, TUNEL staining revealed that KIST0215-1 and KIST0215-2 increased DNA fragmentation compared to control (Figure 4C). Taken together, these results suggest that KIST0215-1 and KIST0215-2 can induce apoptosis and cell cycle arrest in MDA-MB-231 cells.
KIST0215-1 and KIST0215-2 suppress transformation and tumorigenesis of epithelial cells. AP-1 transcription factor activation by various tumour promoters is highly important in the neoplastic transformation of the JB6 C141 cell line. Given that KIST0215-1 and KIST0215-2 reduced the activity of AP-1 in MDA-MB-231 cells, we examined whether these compounds can inhibit the transformation of JB6 C141 cells. Firstly, we examined the effects of the novel compounds on JB6 C141 cell proliferation induced by EGF. Both compounds inhibited EGF-induced cell proliferation (Figure 5A). Secondly, to examine the effect of KIST0215-1 and KIST0215-2 on cell transformation induced by EGF, JB6 Cl41 cells were treated with EGF in the absence or presence of KIST0215-1 and KIST0215-2 in a soft agar matrix and incubated for 14 days. KIST0215-1 and KIST0215-2 significantly reduced EGF-induced colony formation by JB6 C141 cells (Figure 5B and 5C). Finally, we studied the anti-tumorigenic effects of these compounds in vivo. The results showed that KIST0215-1 and KIST0215-2 significantly reduced the size and weight of tumours formed by 4T1 cells in BALB/c mice (Figure 5D).
Discussion
TNBC accounts for 15% of all the breast cancers diagnosed worldwide (26). The pathophysiological features of TNBC are often more aggressive compared to those of other hormone receptor-positive breast cancers, and are represented by high relapse rates and increased metastatic potential (26). TNBC chemotherapy remains challenging since it does not express a distinct therapeutic target (2, 4). The current approach to treat TNBC is focused on the discovery of novel therapeutic targets (5). Pre-clinical trials involving AKT inhibitors that include patasertib have shown promising results against TNBC (27). However, AKT inhibitors have been effective only in combination with the monoclonal antibodies atezolizumab and paclitaxel (27). Similarly, VEGF inhibitors, such as bevacizumab, have failed to improve overall survival in TNBC patients (28). Recently, the United States Food and Drug Administration (USFDA) approved the PARP inhibitor talazoparib for a small subset of TNBC patients having germline mutations in BRCA1/2 (29). The results from these clinical trials clearly suggest that therapeutic targets are still required in TNBC.
Emerging evidence suggests that the MAPK pathway is frequently activated in TNBC that is often accompanied by RAF kinase up-regulation (9, 16). MAPK pathway up-regulation leads to enhanced cell proliferation and survival of TNBC cells. Targeted inhibition of RAF kinases has thus been suggested as a potential chemotherapy option for TNBC patients. Sorafenib inhibits multiple kinases including C-RAF, and has shown promising results against TNBC (5). In a multinational double-blind, randomised phase IIb study evaluating the efficacy and safety of sorafenib in combination with capecitabine (SOLTI-0701 trial), sorafenib significantly improved the median progression-free survival of metastatic breast cancer patients (30). However, the confirmatory phase III, randomised, double-blind RESILIENCE trial comparing sorafenib with capecitabine failed to show similar results (20). Previous studies have shown that all ATP-competitive RAF inhibitors – including sorafenib, dabrafenib and vemurafenib – are poor inhibitors of wild-type B-RAF that paradoxically activate MAPK pathway in B-RAF wild-type cells, including TNBC cells (21, 31). Therefore, significant efforts have been made towards the development of pan-RAF inhibitors for chemotherapy with little attention being paid towards the use of pan-RAF inhibitors in TNBC treatment. We developed two novel imidazo[2,1-b]oxazole compounds, KIST0215-1 and KIST0215-2, as dual inhibitors of B-RAF and C-RAF, which decreased MEK1/2 and ERK1/2 phosphorylation induced by EGF and consequently reduced the EGF-induced proliferation of MDA-MB-231 cells.
Oxazole compounds have significant anti-microbial, anti-viral, and anti-proliferative effects (24). Several oxazole derivatives including linezolid, oxacillin, and sulfisoxazole that have pronounced bioactivity, low toxicity, and excellent pharmacokinetic profiles have been used clinically for a long time (24). A dihydroimidazo[2,1-b]oxazole derivative, delaminid, was recently approved by the USFDA for multi-drug resistant tuberculosis treatment (32). However, the anti-cancer properties of these derivatives are poorly understood. A recent study reported that imidazo[2,1-b]oxazole-based compounds can inhibit RAF kinases, thereby reducing the viability of colon cancer and melanoma cell lines (24). Here, we developed a series of imidazo[2,1-b]oxazole compounds and found that KIST0215-1 and KIST0215-2 are potent inhibitors of B-RAF and C-RAF kinases. We further clarified the anti-cancer properties of these compounds against MDA-MB-231 cells. These novel inhibitors of RAF kinases reduced MEK1/2 and ERK1/2 phosphorylation induced by EGF and consequently reduced c-Fos promoter and AP-1 transactivation activities and prevented the neoplastic transformation of JB6 C141 cells induced by EGF, demonstrating the anti-cancer properties of KIST0215-1 and KIST0215-2 as dual RAF inhibitors.
The AP-1 transcription factor plays an important role in epithelial cell transformation and TNBC tumorigenesis (33, 34). AP-1 composed of a variety of members including c-Fos, c-Jun, activation transcription factor is involved in mediating many biological processes such as proliferation, differentiation and cell death (35). MAPK signalling pathway regulates the function of AP-1 transcription factor (34). Phosphorylation of c-Fos and c-Jun transcription factors by ERKs allows them to dimerise to form a functional AP-1 transcription factor, which modulates the expression of several cancer driver genes including DNA methyltransferase 1, EGFR, cyclin D1, and Bcl2 by binding with TRE/AP-1 response elements found in the promoter region (35, 36). AP-1 family members are significantly up-regulated in TNBC cells and promote the progression of cancer induced by proinflammatory cytokines (33). Furthermore, AP-1 mediated transcription promoted the invasiveness of TNBC cells in vivo in a zebrafish tumour xenograft model (25). Presently, KIST0215-1 and KIST0215-2 reduced the transcriptional activity of AP-1 to prevent epithelial cell transformation and also reduced TNBC cell proliferation.
In summary, the inhibition of RAF kinases using two novel imidazo[2,1-b]oxazole derivatives inhibits epithelial cell transformation and reduces MDA-MB-231 cell tumorigenicity. Furthermore, these novel compounds reduce the growth of tumours formed by 4T1 cells in BALB/c mice. RAF inhibitors are often used in combination with MEK1/2 inhibitors or AKT inhibitors to improve their efficacy and prevent acquired resistance. Therefore, further studies are required to evaluate the efficacy of KIST0215-1 and KIST0215-2 in combination with other compounds, such as MEK inhibitors or AKT inhibitors, to achieve maximum clinical benefit for TNBC patients. Our study not only reports dual inhibitors of RAF kinases but also illustrates the therapeutic potential of dual RAF inhibitors in the chemotherapy of TNBC.
Acknowledgements
This work was supported by research funds from Chosun University (2015).
Footnotes
↵* These Authors contributed equally to this study.
Authors' Contributions
P.Y.B., G.K., M.S.K. and C.H.O. designed and performed experiments, analysed and interpreted data, and wrote the manuscript. H.S.C. conceived the study, designed experiments, interpreted data, and wrote the manuscript. All Authors reviewed the manuscript.
Conflicts of Interest
The Authors declare no conflicts of interest.
- Received May 15, 2020.
- Revision received June 25, 2020.
- Accepted June 30, 2020.
- Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved