Attempt to Construct an In Vitro Model of Enhancement of Macrophage Phagocytosis Via Continuous Administration of LPS

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Figure 1.
Experimental design. ELISA: Enzyme-linked immunosorbent assay; LPS: lipopolysaccharide, Med: Medium, Sup: culture supernatant.
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Figure 2.
Analysis of phagocytic ratio (PR) and phagocytic intensity (PI) of fluorescent latex beads engulfed by RAW264.7 cells treated without (LPS0) or with 100 pg/ml (A), 1 ng/ml (B), or 100 ng/ml (C) of lipopolysaccharide one (LPS1) or three (LPS3) times every 24 h. D: The ratio of PR and PI by LPS1 or LPS3 to that by LPS0 (D). MFI: Mean fluorescent intensity. Values are presented as the mean±SD, n=3. Different letters indicate significant differences at p<0.05.
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Figure 3.
Analysis of cytokines culture supernatant of RAW264.7 cells treated treated without (LPS0) or with 100 pg/ml (A), 1 ng/ml (B), or 100 ng/ml (C) of lipopolysaccharide one (LPS1) or three (LPS3) times every 24 h. Interleukin-6 (IL6) and tumor necrosis factor-α (TNFα) were measured by enzyme-linked immunosorbent assay. D: Increase in cytokine levels by LPS1 and LPS3 relative to that by LPS0. n.d.: Not detected. Values are presented as the mean±SD, n=3. Different letters indicate significant differences at p<0.05.