Abstract
Background/Aim: Time restricted feeding (TRF) mitigates the high-fat diet-enhanced mammary tumorigenesis in a MMTV-PyMT breast cancer model. Materials and Methods: We performed untargeted metabolomic and targeted transcriptomic analyses on mammary tumors from MMTV-PyMT mice fed a standard AIN93G diet, a high-fat diet (HFD), or HFD with TRF (12 h, dark phase) and mammary glands from wild-type mice fed the AIN93G diet. Results: The metabolic profile of mammary tumors differed from that of mammary glands; there was no impact of TRF upon tumor metabolome. TRF did reduce elevated expression of Hmgcr, Srebp1, Fads2, and Ppard in mammary tumors, indicating a down-regulation of lipid metabolism. Conclusion: The null effect of TRF on the metabolomic profile does not rule out changes in more refined intracellular signaling pathways. It suggests that the protection of TRF against mammary tumorigenesis may rely upon its action on the host rather than a direct effect on tumor metabolism.
Footnotes
Authors' Contributions
LY and SS conceived and designed the study, performed the experiments, and collected data. BMR, MJP, and MRB contributed to data analysis and interpretation of results. All Authors contributed to the preparation, review, and revision of the manuscript. All Authors agreed to be accountable for the content of the work.
This article is freely accessible online.
Conflicts of Interest
The Authors have declared that no competing interests exist regarding this study.
Funding
This work was funded by the USDA Agricultural Research Service Projects #3062-51000-050-00D and #3062-51000-056-00D (LY) and #3062-53000-001-00D (MJP).
- Received May 2, 2020.
- Revision received May 16, 2020.
- Accepted May 20, 2020.
- Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved