Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Anticancer Research
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Anticancer Research

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Visit us on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

KHYG-1 Cells With EGFRvIII-specific CAR Induced a Pseudoprogression-like Feature in Subcutaneous Tumours Derived from Glioblastoma-like Cells

TSUTOMU NAKAZAWA, TOSHIHARU MURAKAMI, ATSUSHI NATSUME, FUMIHIKO NISHIMURA, TAKAYUKI MORIMOTO, RYOSUKE MATSUDA, MITSUTOSHI NAKAMURA, SHUICHI YAMADA, ICHIRO NAKAGAWA, YOUNG-SOO PARK, YASUSHI MOTOYAMA, TAKAHIRO TSUJIMURA, TOSHIHIKO WAKABAYASHI and HIROYUKI NAKASE
Anticancer Research June 2020, 40 (6) 3231-3237; DOI: https://doi.org/10.21873/anticanres.14304
TSUTOMU NAKAZAWA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
2Grandsoul Research Institute for Immunology, Inc., Uda, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: nakazawa@naramed-u.ac.jp
TOSHIHARU MURAKAMI
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
ATSUSHI NATSUME
3Department of Neurosurgery, Nagoya University, Graduate School of Medicine, Nagoya, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
FUMIHIKO NISHIMURA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TAKAYUKI MORIMOTO
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
RYOSUKE MATSUDA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MITSUTOSHI NAKAMURA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
4Clinic Grandsoul Nara, Uda, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SHUICHI YAMADA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
ICHIRO NAKAGAWA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YOUNG-SOO PARK
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YASUSHI MOTOYAMA
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TAKAHIRO TSUJIMURA
4Clinic Grandsoul Nara, Uda, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TOSHIHIKO WAKABAYASHI
3Department of Neurosurgery, Nagoya University, Graduate School of Medicine, Nagoya, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
HIROYUKI NAKASE
1Department of Neurosurgery, Nara Medical University, Kashihara, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Background/Aim: We previously established a novel type of epidermal growth factor receptor variant III (EGFRvIII)-specific chimeric antigen receptor (CAR)-expressing natural killer (NK) cell line, designated EvCAR-KHYG-1, which inhibited the growth of glioblastoma (GBM) cells in vitro via apoptosis. Materials and Methods: We investigated the cytokine-producing effect of EvCAR-KHYG-1 cells on GBM-like cell lines and their antitumour effect using in vivo xenograft assays. Results: EvCAR-KHYG-1 cells produced interleukin-2, interferon-γ, and tumour necrosis factor-α on EGFRvIII-expressing U87MG cells. In vivo xenograft assays showed that EvCAR-KHYG-1 cells did not reduce the volume of subcutaneous tumours derived from EGFRvIII-expressing U87MG cells but did reduce tumour cell occupancy. Conclusion: EvCAR-KHYG-1 cells led to expression of cellular immunity-related cytokines on EGFRvIII-expressing U87MG in vitro but did not inhibit tumour progression due to the induction of a pseudo progression-like pathological feature. Future studies investigating the effect of different conditions in vivo are required to study the inhibition of tumour progression in GBM.

  • Chimeric antigen receptor
  • natural killer cells
  • glioblastoma
  • epidermal growth factor receptor variant III
  • Received April 30, 2020.
  • Revision received May 14, 2020.
  • Accepted May 15, 2020.
  • Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
View Full Text

This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.

patientACCESS

patientACCESS - Patients desiring access to articles
PreviousNext
Back to top

In this issue

Anticancer Research: 40 (6)
Anticancer Research
Vol. 40, Issue 6
June 2020
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Anticancer Research.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
KHYG-1 Cells With EGFRvIII-specific CAR Induced a Pseudoprogression-like Feature in Subcutaneous Tumours Derived from Glioblastoma-like Cells
(Your Name) has sent you a message from Anticancer Research
(Your Name) thought you would like to see the Anticancer Research web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
5 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
KHYG-1 Cells With EGFRvIII-specific CAR Induced a Pseudoprogression-like Feature in Subcutaneous Tumours Derived from Glioblastoma-like Cells
TSUTOMU NAKAZAWA, TOSHIHARU MURAKAMI, ATSUSHI NATSUME, FUMIHIKO NISHIMURA, TAKAYUKI MORIMOTO, RYOSUKE MATSUDA, MITSUTOSHI NAKAMURA, SHUICHI YAMADA, ICHIRO NAKAGAWA, YOUNG-SOO PARK, YASUSHI MOTOYAMA, TAKAHIRO TSUJIMURA, TOSHIHIKO WAKABAYASHI, HIROYUKI NAKASE
Anticancer Research Jun 2020, 40 (6) 3231-3237; DOI: 10.21873/anticanres.14304

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
KHYG-1 Cells With EGFRvIII-specific CAR Induced a Pseudoprogression-like Feature in Subcutaneous Tumours Derived from Glioblastoma-like Cells
TSUTOMU NAKAZAWA, TOSHIHARU MURAKAMI, ATSUSHI NATSUME, FUMIHIKO NISHIMURA, TAKAYUKI MORIMOTO, RYOSUKE MATSUDA, MITSUTOSHI NAKAMURA, SHUICHI YAMADA, ICHIRO NAKAGAWA, YOUNG-SOO PARK, YASUSHI MOTOYAMA, TAKAHIRO TSUJIMURA, TOSHIHIKO WAKABAYASHI, HIROYUKI NAKASE
Anticancer Research Jun 2020, 40 (6) 3231-3237; DOI: 10.21873/anticanres.14304
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • The Mechanism of the Synergistic Anticancer Effect of CDDP and EPA in the TE1 Cell Line
  • Ephrin Receptor A4 Expression Enhances Migration, Invasion and Neurotropism in Pancreatic Ductal Adenocarcinoma Cells
  • Oral-recombinant Methioninase Converts an Osteosarcoma from Docetaxel-resistant to -Sensitive in a Clinically-relevant Patient-derived Orthotopic-xenograft (PDOX) Mouse Model
Show more Experimental Studies

Similar Articles

Keywords

  • Chimeric antigen receptor
  • Natural killer cells
  • glioblastoma
  • epidermal growth factor receptor variant III
Anticancer Research

© 2021 Anticancer Research

Powered by HighWire