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Research ArticleExperimental Studies

Galectin-8 Favors VEGF-Induced Angiogenesis: In Vitro Study in Human Umbilical Vein Endothelial Cells and In Vivo Study in Chick Chorioallantoic Membrane

LENKA VARINSKÁ, LENKA FÁBER, EVA PETROVOVÁ, LUDMILA BALÁŽOVÁ, ELEONÓRA IVANČOVÁ, MICHAL KOLÁŘ and PETER GÁL
Anticancer Research June 2020, 40 (6) 3191-3201; DOI: https://doi.org/10.21873/anticanres.14300
LENKA VARINSKÁ
1Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovak Republic
2Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic
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LENKA FÁBER
1Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovak Republic
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EVA PETROVOVÁ
3Department of Anatomy, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
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LUDMILA BALÁŽOVÁ
4Department of Pharmacognosy and Botany, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
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ELEONÓRA IVANČOVÁ
5Department of Stomatology and Maxilofacial Surgery, Pavol Jozef Šafárik University and Louis Pasteur University Hospital, Košice, Slovak Republic
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MICHAL KOLÁŘ
6Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
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PETER GÁL
2Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic
7Laboratory of Cell Interactions, MediPark, Pavol Jozef Šafárik University, Košice, Slovak Republic
8Prague Burn Center, Third Faculty of Medicine, Charles University, Prague, Czech Republic
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  • For correspondence: pgal@vusch.sk galovci@yahoo.com
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Abstract

Background/Aim: Although it has been accepted that the tandem repeat galectin-8 (Gal-8) is linked to angiogenesis, the underlying mechanisms in endothelial cells has remained poorly understood. In this study we aimed to investigate the effect of Gal-8 on selected biological processes linked to angiogenesis in in vitro and in vivo models. Materials and Methods: In detail, we assessed how exogenously added human recombinant Gal-8 (with or without vascular endothelial growth factor – VEGF) affects selected steps involved in vessel formation in human umbilical vein endothelial cells (HUVECs) as well as using the chick chorioallantoic membrane (CAM) assay. Gene expression profiling of HUVECs was performed to extend the scope of our investigation. Results: Our findings demonstrate that Gal-8 in combination with VEGF enhanced cell proliferation and migration, two cellular events linked to angiogenesis. However, Gal-8 alone did not exhibit any significant effects on cell proliferation or on cell migration. The molecular analysis revealed that Gal-8 in the presence of VEGF influenced cytokine-cytokine receptor interactions, HIF-1 and PI3K/AKT signaling pathways. Gal-8 alone also targeted cytokine-cytokine receptor interactions, but with a different expression profile as well as a modulated focal adhesion and TNF signaling. Conclusion: Gal-8 promotes a pro-angiogenic phenotype possibly in a synergistic manner with VEGF.

  • Tumor growth
  • wound healing
  • vessel sprouting
  • glycobiology
  • sugar code
  • Received April 6, 2020.
  • Revision received April 20, 2020.
  • Accepted April 23, 2020.
  • Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
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Anticancer Research: 40 (6)
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Galectin-8 Favors VEGF-Induced Angiogenesis: In Vitro Study in Human Umbilical Vein Endothelial Cells and In Vivo Study in Chick Chorioallantoic Membrane
LENKA VARINSKÁ, LENKA FÁBER, EVA PETROVOVÁ, LUDMILA BALÁŽOVÁ, ELEONÓRA IVANČOVÁ, MICHAL KOLÁŘ, PETER GÁL
Anticancer Research Jun 2020, 40 (6) 3191-3201; DOI: 10.21873/anticanres.14300

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Galectin-8 Favors VEGF-Induced Angiogenesis: In Vitro Study in Human Umbilical Vein Endothelial Cells and In Vivo Study in Chick Chorioallantoic Membrane
LENKA VARINSKÁ, LENKA FÁBER, EVA PETROVOVÁ, LUDMILA BALÁŽOVÁ, ELEONÓRA IVANČOVÁ, MICHAL KOLÁŘ, PETER GÁL
Anticancer Research Jun 2020, 40 (6) 3191-3201; DOI: 10.21873/anticanres.14300
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Keywords

  • Tumor growth
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  • glycobiology
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