Establishment and Characterization of a Novel Primitive Yolk Sac Tumour Cell Line, TC587

Abstract

Background/Aim: Yolk sac tumour (YST) is a rare malignant ovarian germ cell tumour that often occurs in young women or adolescents and exhibits an unfavourable outcome. To evaluate the biological behavior of carcinomas in vitro, permanent tumour cell lines are required. However, previously, only a few human YST cell lines have been established. Therefore, we aimed to establish a novel YST cell line. Materials and Methods: We established a novel YST cell line, TC587, from an adolescent patient with ovarian YST. Results: The cell line expressed AFP and SALL4, the characteristics of YST. In addition, we evaluated somatic mutations using next-generation sequencing and revealed some pathogenic variants, including mutations in the NRAS, KIT, KMT2C, RSF1, and TP53 genes. Conclusion: The newly established TC587 cell line may represent an effective tool for developing treatments and conducting molecular analyses for YST.