Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Anticancer Research
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Anticancer Research

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Visit us on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

The Significant Association of MMP-1 Genotypes With Taiwan Pterygium

CHONG-BIN TSAI, NING-YI HSIA, YUN-CHI WANG, ZHI-HONG WANG, YU-TING CHIN, TAI-LIN HUANG, CHIEN-CHIH YU, WEN-SHIN CHANG, CHIA-WEN TSAI, MEI-CHIN YIN and DA-TIAN BAU
Anticancer Research February 2020, 40 (2) 703-707; DOI: https://doi.org/10.21873/anticanres.14000
CHONG-BIN TSAI
1Department of Ophthalmology, Ditmanson Medical Foundation, Chia-Yi Christian Hospital, Chia-Yi, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
NING-YI HSIA
2Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan, R.O.C.
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YUN-CHI WANG
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
ZHI-HONG WANG
4Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YU-TING CHIN
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TAI-LIN HUANG
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CHIEN-CHIH YU
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
5School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
WEN-SHIN CHANG
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CHIA-WEN TSAI
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: datian@mail.cmuh.org.tw artbau2@gmail.com
MEI-CHIN YIN
4Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: datian@mail.cmuh.org.tw artbau2@gmail.com
DA-TIAN BAU
3Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
6Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: datian@mail.cmuh.org.tw artbau2@gmail.com
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Background/Aim: Few studies have examined the contribution of matrix metalloproteinases (MMP) to either diagnosis or prognosis of pterygium. The aim of this study was to investigate the contribution of MMP-1 genotypes to pterygium risk. Patients and Methods: A total of 134 cases and 268 controls were included and their MMP-1 -1607 (rs1799705) genotypes were examined by polymerase chain reaction-restriction fragment length polymorphism. Results: The percentages of 2G/2G, 1G/2G, and 1G/1G for rs1799705 genotypes were 48.5, 36.6 and 14.9% among patients and 33.9, 44.8, and 21.3% among controls (p trend=0.0167). The odds ratios (ORs) after adjusting for age and gender for 1G/2G and 1G/1G genotypes at rs1799705 were 0.54 (95%CI=0.33-0.89, p=0.0168) and 0.46 (95%CI=0.27-0.88, p=0.0192), respectively. Consistently, the adjusted OR for those carrying the 1G allele at MMP-1 -1607 was 0.61 (95%CI=0.41-0.78, p=0.0167), compared with the wild-type 2G allele. Conclusion: The genotypes at rs1799705 play a role in determining personal susceptibility to pterygium.

  • Age
  • gender
  • genotype
  • MMP-1
  • polymorphism
  • pterygium
  • Taiwan

Pterygium is the formation of fibrovascular tissues consisting of highly vascularized epithelial and subepithelial cells that proliferate excessively and generate an abnormal wing-shaped growth from the conjunctiva over the ocular surface (1). Epidemiologically, population-based reports have shown that heat, dust, particles in the atmosphere, immunological mechanisms, and agents inducing extracellular matrix reorganization, growth factors, cytokines, and those affect apoptosis and angiogenesis are risk factors for pterygium (2-10). In recent decades, several reports have demonstrated that inherited genomic variants play a significant role in the determination of individual susceptibility to pterygium (11-14).

Matrix metalloproteinases (MMPs), also known as matrixins or matrix metallopeptidases, comprise the major calcium-dependent zinc-containing protein family responsible for regulating the components of extracellular matrix (15). MMPs are also function in cell proliferation, differentiation, apoptosis, invasion, adhesion, dispersion, migration, angiogenesis and immune surveillance (16, 17). It has been shown that polymorphic genotypes of MMPs may associate with the personal susceptibility to several types of cancer (18-21). However, the obstacle in recruiting pterygium patients and matched controls has retarded the progress in deciphering the role of MMPs in pterygium etiology and only limited reports with a small size of pterygium samples have provided evidence for MMPs' involvements in the initiation and progression of pterygium (22-24).

The most commonly studied MMP-1 polymorphic site is the rs1799750, which is located at -1607 of the promoter of the MMP-1 gene. The variants at this polymorphic site may be consist of the “2G” insertion polymorphism, which has been reported to lead to higher transcriptional activity of MMP-1, potentially to higher levels or rates of collagen breakdown, and higher levels of MMP-1 in the serum than the 1G/1G genotype (25). In a meta-analysis published in 2012 investigating about 10,000 cancer cases, half of which metastasized, concluded that MMP-1 rs1799750 2G/2G genotypes carriers may have a slightly higher overall metastasis rate (26). As far as pterygium is concerned, MMP-1 and MMP-3 have been reported to be overexpressed at the levels of both mRNA and protein in Pterygium head fibroblasts (22). Thus, it is reasonable to hypothesize that the variant genotypes at the promoter region at MMP-1 rs1799750 may play a role in determining the expression levels of MMP-1, and personal susceptibility for pterygium. Since the promoter polymorphic site of MMP-1 may control the expression levels of MMP-1 and consequently the levels of extracellular matrix components, we aimed at investigating the association of MMP-1 rs1799750 genotypes with the susceptibility of pterygium in Taiwan.

Materials and Methods

Collection of pterygium patients and matched controls. The protocols of the study were approved by the Institutional Review Board of Changhua Christian Hospital and written informed consent was obtained from one or both parents of all participants (Changhua Christian Hospital IRB numbers: 151225). Totally, 134 cases diagnosed with pterygium were recruited in this study. They voluntarily participated, completed a questionnaire, and provided their peripheral blood samples. At the same time, healthy subjects, aged 45 years or more without pterygium or any type of cancer were enrolled as controls. There were 78 males and 56 females in the pterygium group (age range between 48-89 years and average age of 64.4 years). At last, 268, double the number of the cases, healthy participants were included into the control group matching the population structure (matched for their ages with difference no larger than 5 years and genders). Selected characteristics of the participants both in pterygium and control groups are summarized and compared in Table I.

MMP-1 rs1799750 genotyping. The genomic DNA from peripheral blood leukocytes of each subject was extracted, aliquoted and stored in −80°C as previously described (13, 14). The MMP-1 genotyping methodology is the same as described in our recently published papers (19, 27). The polymerase chain reaction (PCR) protocols set at My Cycler (Biorad, Hercules, CA, USA) for MMP-1 were one cycle at 94°C for 5 min; 35 cycles at 94°C for 30 s, one cycle at 57°C for 30 s and one cycle at 72°C for 30 s and a final extension at 72°C for 10 min.

Statistical analysis. Typical Pearson's Chi-square test without Yates' correction (when all numbers were equal to or larger than 5) and Fisher's exact test (when any number was less than 5) was applied to compare the distribution of the gender, MMP-1 genotypic and allelic distributions between pterygium and control groups. Also, the unpaired Student's t-test was applied for the comparison of distribution of the ages between the case and control groups. In addition, the associations between the MMP-1 polymorphisms and pterygium risk were estimated by computing odds ratios (ORs) as well as their 95% confidence intervals (CIs) from unconditional logistic regression analysis with the adjustment for possible confounding factors including age and gender (Tables II and III).

Results

Well-match of ages and genders between the pterygium patient and control groups. The distributions of the age and gender for the 134 pterygium patients and the 268 non-pterygium controls are shown and compared in Table I. The average onset age of the pterygium patients was 64.4 years old, and the percentages of males and females were 58.2% and 41.8% in control and case groups, respectively. Since we originally matched the age and gender in our research design, there was no significant difference between the two groups as for the frequencies of age or gender as expected (both p>0.05) (Table I).

Association analysis of MMP-1 promoter genotypes at rs1799750 with pterygium risk. The results of genotypic analysis of the MMP-1 promoter rs1799750 polymorphism among the pterygium cases and non-pterygium controls are presented and compared in Table II. First, the genotypic frequency distributions at MMP-1 rs1799750 were statistically different between the pterygium and control groups (p for trend=0.0167) (Table II, top panel). In detail, the MMP-1 rs1799750 heterozygous 1G/2G and homozygous 1G/1G variant genotypes were both associated with lower risk for pterygium than the wild-type 2G/2G genotype among the investigated population (p=0.0168 and 0.0192, adjusted OR=0.54 and 0.46, 95%CI=0.33-0.89 and 0.27-0.88, respectively; Table II, top panel). In the recessive model, the combination of the wild-type 2G/2G and heterozygote 1G/2G genotypes (2G/2G+1G/2G) at MMP-1 rs1799750 conferred an unaltered risk for pterygium compared to 1G/1G genotype (p=0.1276) (Table II, middle panel). In the dominant model, the 1G/1G+1G/2G carriers at MMP-1 rs1799750 conferred lower risk of pterygium compared to the 2G/2G genotype carriers (p=0.0048, aOR=0.53 and 95%CI=0.35-0.81) (Table II, bottom panel). Overall, the MMP-1 rs1799750 genotypes play a critical role in determining personal susceptibility to pterygium among Taiwanese.

Association analysis of MMP-1 rs1799750 allelic frequencies with pterygium risk. The allelic frequency analysis of MMP-1 rs1799750 with pterygium risk was also conducted to confirm the findings in Table II. The results are shown in Table III. Consistent with the major finding in Table II, there is a significant difference in the distribution of allelic frequencies between the pterygium and control groups regarding MMP-1 rs1799750 (Table III). In detail, the adjusted OR for the subjects carrying the variant 1G allele at MMP-1 rs1799750 was 0.61 (95%CI=0.41-0.78, p=0.0167), compared to those carrying the wild-type 2G allele (Table III).

Discussion

MMPs play a critical role in the homeostasis of extracellular matrix components, and any imbalance of the extracellular microenvironment may be related to the initiation and progression of pterygium. It has been reported that in early-stage pterygium the levels of MMP-2 or MMP-9 were low or undetectable in tissues and cultured fibroblasts. On the contrary, in advanced-stage pterygium, the levels of MMP-2 and MMP-9 were higher in pterygium tissues and fibroblasts (28). In addition, Kim and his colleagues have reported that down-regulation of the expression levels of MMP-3 and MMP-13 resulted in suppression of the proliferation and migration of pterygium fibroblasts (29). These findings support the idea that MMPs may play an important role in the progression of pterygium, and polymorphisms of MMPs may serve as valuable predictive biomarkers for the risk of pterygium.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table I.

Distribution of selected demographics of the 134 pterygium patients and the 268 non-pterygium controls.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table II.

Distributions of matrix metalloproteinase-1 rs1799750 genotypic frequencies among the pterygium patients and healthy controls.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table III.

Allelic frequencies for matrix metalloproteinase-1 rs1799750 polymorphisms among the pterygium patients and healthy controls.

The positive association of MMP-1 rs1799750 genotypes with pterygium in the current study (Tables II and III), supports the concept that polymorphic variations in MMP-1 promoter region may influence personal susceptibility to pterygium by regulating MMP-1 expression at the transcription level. This is consistent with the findings in childhood leukemia (30), and gastric cancer (31). However, in several types of cancer, the genotypes of MMP-1 rs1799750 may not directly contribute to the risk determination (20, 32-34), which indicates that the MMP-1 rs1799750 genotypes may be indirectly involved in carcinogenesis. The detailed mechanisms of how MMP-1 rs1799750 genotypes interact with other molecules leading to pterygium need further investigation.

In conclusion, this is the first study that has provided evidence on the association of polymorphisms at MMP-1 rs1799750 with pterygium. Our results suggest that the variant genotypes of the rs1799750 at the promoter of MMP-1 significantly confer personal susceptibility to pterygium among Taiwanese. Further studies elucidating the contribution of the genotypes of other members of MMPs, such as MMP-2, -9 (28), -3, -13 (29) and -27, to pterygium are needed and the findings in the current study should be validated in other studies to strengthen their value.

Acknowledgements

The Authors are grateful to Hsin-Ting Li, Yu-Chen Hsiau, Yu-Hsin Lin and Yi-Ru Huang for their excellent technical assistance. All the participants including those who were not selected into the control group of the study are appreciated. This study was supported mainly by Chia-Yi Christian Hospital, Chia-Yi, Taiwan to Dr. Tsai (grant number: I108HA131). The funders had no role in study design, data collection, statistical analysis, or decision to publish or preparation of the manuscript.

Footnotes

  • Authors' Contributions

    Research design: Tsai CB, Wang YC and Yin MC; patient and questionnaire summaries: Tsai CB and Hsia NY; experimental work: Wang YC, CHIN YC and Huang TL; statistical analysis: Wang ZH, Chang WS and Yu CC; article writing: Tsai CW and Bau DT; review and revision: Chang WS, Tsai CW and Bau DT.

  • Conflicts of Interest

    All the Authors have declared no conflicts of interest regarding this study.

  • Received January 7, 2020.
  • Revision received January 10, 2020.
  • Accepted January 13, 2020.
  • Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved

References

  1. ↵
    1. Liu L,
    2. Wu J,
    3. Geng J,
    4. Yuan Z,
    5. Huang D
    : Geographical prevalence and risk factors for pterygium: a systematic review and meta-analysis. BMJ Open 3: e003787, 2013. PMID: 24253031. DOI: 10.1136/bmjopen-2013-003787
    OpenUrlAbstract/FREE Full Text
  2. ↵
    1. Asokan R,
    2. Venkatasubbu RS,
    3. Velumuri L,
    4. Lingam V,
    5. George R
    : Prevalence and associated factors for pterygium and pinguecula in a South Indian population. Ophthalmic Physiol Opt 32: 39-44, 2012. PMID: 22112236. DOI: 10.1111/j.1475-1313.2011.00882.x
    OpenUrlCrossRefPubMed
    1. Viso E,
    2. Gude F,
    3. Rodriguez-Ares MT
    : Prevalence of pinguecula and pterygium in a general population in Spain. Eye (Lond) 25: 350-357, 2011. PMID: 21183945. DOI: 10.1038/eye.2010.204
    OpenUrl
    1. Liang QF,
    2. Xu L,
    3. Jin XY,
    4. You QS,
    5. Yang XH,
    6. Cui TT
    : Epidemiology of pterygium in aged rural population of Beijing, China. Chin Med J (Engl) 123: 1699-1701, 2010. PMID: 20819632.
    OpenUrlPubMed
    1. Cajucom-Uy H,
    2. Tong L,
    3. Wong TY,
    4. Tay WT,
    5. Saw SM
    : The prevalence of and risk factors for pterygium in an urban Malay population: the Singapore Malay Eye Study (SiMES). Br J Ophthalmol 94: 977-981, 2010. PMID: 19965830. DOI: 10.1136/bjo.2008.150847
    OpenUrlAbstract/FREE Full Text
    1. West S,
    2. Munoz B
    : Prevalence of pterygium in Latinos: Proyecto VER. Br J Ophthalmol 93: 1287-1290, 2009. PMID: 19570772. DOI: 10.1136/bjo.2008.152694
    OpenUrlAbstract/FREE Full Text
    1. Shiroma H,
    2. Higa A,
    3. Sawaguchi S,
    4. Iwase A,
    5. Tomidokoro A,
    6. Amano S,
    7. Araie M
    : Prevalence and risk factors of pterygium in a southwestern island of Japan: the Kumejima Study. Am J Ophthalmol 148: 766-771 e761, 2009. PMID: 19664753. DOI: 10.1016/j.ajo.2009.06.006
    OpenUrlCrossRefPubMed
    1. Fotouhi A,
    2. Hashemi H,
    3. Khabazkhoob M,
    4. Mohammad K
    : Prevalence and risk factors of pterygium and pinguecula: the Tehran Eye Study. Eye (Lond) 23: 1125-1129, 2009. PMID: 18600244. DOI: 10.1038/eye.2008.200
    OpenUrl
    1. Durkin SR,
    2. Abhary S,
    3. Newland HS,
    4. Selva D,
    5. Aung T,
    6. Casson RJ
    : The prevalence, severity and risk factors for pterygium in central Myanmar: the Meiktila Eye Study. Br J Ophthalmol 92: 25-29, 2008. PMID: 18055574. DOI: 10.1136/bjo.2007.119842
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Nemesure B,
    2. Wu SY,
    3. Hennis A,
    4. Leske MC,
    5. Barbados Eye Studies G
    : Nine-year incidence and risk factors for pterygium in the barbados eye studies. Ophthalmology 115: 2153-2158, 2008. PMID: 18930552. DOI: 10.1016/j.ophtha.2008.08.003
    OpenUrlCrossRefPubMed
  4. ↵
    1. Tsai YY,
    2. Bau DT,
    3. Chiang CC,
    4. Cheng YW,
    5. Tseng SH,
    6. Tsai FJ
    : Pterygium and genetic polymorphism of DNA double strand break repair gene Ku70. Mol Vis 13: 1436-1440, 2007. PMID: 17768380.
    OpenUrlPubMed
    1. Bau DT,
    2. Chiang CC,
    3. Tsai YY,
    4. Lee CC,
    5. Tsai Y,
    6. Lin CC,
    7. Tsai CH,
    8. Tsai FJ
    : Evaluation of transforming growth factor and vascular endothelial growth factor polymorphisms in Taiwan Chinese patients with pterygium. Eur J Ophthalmol 18: 21-26, 2008. PMID: 18203080. DOI: 10.1177/112067210801800104
    OpenUrlPubMed
  5. ↵
    1. Hu PS,
    2. Chang WS,
    3. Chou AK,
    4. Hsia NY,
    5. Hung YW,
    6. Lin CW,
    7. Wu CW,
    8. Huang CY,
    9. Wu MF,
    10. Liao CH,
    11. Tsai CW,
    12. Bau DT,
    13. Gong CL
    : The association of MMP-8 genotypes with pterygium. In Vivo 32: 41-46, 2018. PMID: 29275297. DOI: 10.21873/invivo.11202
    OpenUrlAbstract/FREE Full Text
  6. ↵
    1. Hu PS,
    2. Wang YC,
    3. Liao CH,
    4. Hsia NY,
    5. Wu MF,
    6. Yang JS,
    7. Yu CC,
    8. Chang WS,
    9. Bau DT,
    10. Tsai CW
    : The association of MMP7 genotype with pterygium. In Vivo 34: 51-56, 2020. PMID: 31882462. DOI: 10.21873/invivo.11744
    OpenUrlAbstract/FREE Full Text
  7. ↵
    1. de Souza AP,
    2. Trevilatto PC,
    3. Scarel-Caminaga RM,
    4. Brito RB,
    5. Line SR
    : MMP-1 promoter polymorphism: association with chronic periodontitis severity in a Brazilian population. J Clin Periodontol 30: 154-158, 2003. PMID: 12622858. DOI: 10.1034/j.1600-051x.2003.300202.x
    OpenUrlCrossRefPubMed
  8. ↵
    1. Egeblad M,
    2. Werb Z
    : New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer 2: 161-174, 2002. PMID: 11990853. DOI: 10.1038/nrc745
    OpenUrlCrossRefPubMed
  9. ↵
    1. Van Lint P,
    2. Libert C
    : Chemokine and cytokine processing by matrix metalloproteinases and its effect on leukocyte migration and inflammation. J Leukoc Biol 82: 1375-1381, 2007. PMID: 17709402. DOI: 10.1189/jlb.0607338
    OpenUrlCrossRefPubMed
  10. ↵
    1. Wu MH,
    2. Tzeng HE,
    3. Wu CN,
    4. Yueh TC,
    5. Peng YC,
    6. Tsai CH,
    7. Wang YC,
    8. Ke TW,
    9. Pei JS,
    10. Chang WS,
    11. Tsai CW,
    12. Bau DT
    : Association of matrix metalloproteinase-9 rs3918242 promoter genotypes with colorectal cancer risk. Anticancer Res 39: 6523-6529, 2019. PMID: 31810917. DOI: 10.21873/anticanres.13867
    OpenUrlAbstract/FREE Full Text
  11. ↵
    1. Liao CH,
    2. Wu HC,
    3. Hu PS,
    4. Hsu SW,
    5. Shen TC,
    6. Hsia TC,
    7. Chang WS,
    8. Tsai CW,
    9. Bau DT
    : The Association of matrix metalloproteinase-1 promoter polymorphisms with prostate cancer in Taiwanese patients. Anticancer Res 38: 3907-3911, 2018. PMID: 29970511. DOI: 10.21873/anticanres.12675
    OpenUrlAbstract/FREE Full Text
  12. ↵
    1. Yueh TC,
    2. Wu CN,
    3. Hung YW,
    4. Chang WS,
    5. Fu CK,
    6. Pei JS,
    7. Wu MH,
    8. Lai YL,
    9. Lee YM,
    10. Yen ST,
    11. Li HT,
    12. Tsai CW,
    13. Bau DT
    : The contribution of MMP-7 genotypes to colorectal cancer susceptibility in Taiwan. Cancer Genomics Proteomics 15: 207-212, 2018. PMID: 29695403. DOI: 10.21873/cgp.20079
    OpenUrlAbstract/FREE Full Text
  13. ↵
    1. Shen TC,
    2. Chang WS,
    3. Tsai CW,
    4. Chao CY,
    5. Lin YT,
    6. Hsiao CL,
    7. Hsu CL,
    8. Chen WC,
    9. Hsia TC,
    10. Bau DT
    : The Contribution of matrix metalloproteinase-1 promoter genotypes in Taiwan lung cancer risk. Anticancer Res 38: 253-257, 2018. PMID: 29277780. DOI: 10.21873/anticanres.12215
    OpenUrlAbstract/FREE Full Text
  14. ↵
    1. Li DQ,
    2. Lee SB,
    3. Gunja-Smith Z,
    4. Liu Y,
    5. Solomon A,
    6. Meller D,
    7. Tseng SC
    : Overexpression of collagenase (MMP-1) and stromelysin (MMP-3) by pterygium head fibroblasts. Arch Ophthalmol 119: 71-80, 2001. PMID: 11146729.
    OpenUrlCrossRefPubMed
    1. Schellini SA,
    2. Hoyama E,
    3. Oliveira DE,
    4. Bacchi CE,
    5. Padovani CR
    : Matrix metalloproteinase-9 expression in pterygium. Arq Bras Oftalmol 69: 161-164, 2006. PMID: 16699663. DOI: 10.1590/s0004-27492006000200005
    OpenUrlCrossRefPubMed
  15. ↵
    1. Hasty KA,
    2. Pourmotabbed TF,
    3. Goldberg GI,
    4. Thompson JP,
    5. Spinella DG,
    6. Stevens RM,
    7. Mainardi CL
    : Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J Biol Chem 265: 11421-11424, 1990. PMID: 2164002.
    OpenUrlAbstract/FREE Full Text
  16. ↵
    1. Tower GB,
    2. Coon CI,
    3. Brinckerhoff CE
    : The 2G single nucleotide polymorphism (SNP) in the MMP-1 promoter contributes to high levels of MMP-1 transcription in MCF-7/ADR breast cancer cells. Breast Cancer Res Treat 82: 75-82, 2003. PMID: 14692651. DOI: 10.1023/B:BREA.0000003948.14026.7c
    OpenUrlCrossRefPubMed
  17. ↵
    1. Liu D,
    2. Guo H,
    3. Li Y,
    4. Xu X,
    5. Yang K,
    6. Bai Y
    : Association between polymorphisms in the promoter regions of matrix metalloproteinases (MMPs) and risk of cancer metastasis: a meta-analysis. PLoS One 7: e31251, 2012. PMID: 22348060. DOI: 10.1371/journal.pone.0031251
    OpenUrlCrossRefPubMed
  18. ↵
    1. Hsiao CL,
    2. Liu LC,
    3. Shih TC,
    4. Lai YL,
    5. Hsu SW,
    6. Wang HC,
    7. Pan SY,
    8. Shen TC,
    9. Tsai CW,
    10. Chang WS,
    11. Su CH,
    12. Way TD,
    13. Chung JG,
    14. Bau DT
    : The association of matrix metalloproteinase-1 promoter polymorphisms with breast cancer. In Vivo 32: 487-491, 2018. PMID: 29695550. DOI: 10.21873/invivo.11265
    OpenUrlAbstract/FREE Full Text
  19. ↵
    1. Yang SF,
    2. Lin CY,
    3. Yang PY,
    4. Chao SC,
    5. Ye YZ,
    6. Hu DN
    : Increased expression of gelatinase (MMP-2 and MMP-9) in pterygia and pterygium fibroblasts with disease progression and activation of protein kinase C. Invest Ophthalmol Vis Sci 50: 4588-4596, 2009. PMID: 19420332. DOI: 10.1167/iovs.08-3147
    OpenUrlAbstract/FREE Full Text
  20. ↵
    1. Kim YH,
    2. Jung JC,
    3. Gum SI,
    4. Park SB,
    5. Ma JY,
    6. Kim YI,
    7. Lee KW,
    8. Park YJ
    : Inhibition of Pterygium fibroblast migration and outgrowth by bevacizumab and cyclosporine a involves down-regulation of matrix metalloproteinases-3 and -13. PLoS One 12: e0169675, 2017. PMID: 28068383. DOI: 10.1371/journal. pone.0169675
    OpenUrl
  21. ↵
    1. Pei JS,
    2. Hsu PC,
    3. Chou AK,
    4. Tsai CW,
    5. Chang WS,
    6. Hsiao CL,
    7. Hsu YN,
    8. Cheng SP,
    9. Bau DT
    : Matrix metalloproteinase-1 genotype contributes to the risk of non-solid tumor in childhood leukemia. Anticancer Res 36: 5127-5132, 2016. PMID: 27798872. DOI: 10.21873/anticanres.11082
    OpenUrlAbstract/FREE Full Text
  22. ↵
    1. Devulapalli K,
    2. Bhayal AC,
    3. Porike SK,
    4. Macherla R,
    5. Akka J,
    6. Nallari P,
    7. Ananthapur V
    : Role of interstitial collagenase gene promoter polymorphism in the etiology of gastric cancer. Saudi J Gastroenterol 20: 309-314, 2014. PMID: 25253367. DOI: 10.4103/1319-3767.141693
    OpenUrl
  23. ↵
    1. Zhou H,
    2. Zhu X
    : Association between matrix-metalloproteinase polymorphisms and prostate cancer risk: a meta-analysis and systematic review. Cancer Manag Res 10: 5247-5259, 2018. PMID: 30464622. DOI: 10.2147/CMAR. S177551
    OpenUrl
    1. Zhu XM,
    2. Sun WF
    : Association between matrix metalloproteinases polymorphisms and ovarian cancer risk: A meta-analysis and systematic review. PLoS One 12: e0185456, 2017. PMID: 28957437. DOI: 10.1371/journal.pone.0185456
    OpenUrlCrossRef
  24. ↵
    1. Wang L,
    2. Kong B
    : Analysis of the association of matrix metalloproteinase-1 gene promoter (rs1799750) polymorphism and risk of ovarian cancer. Int J Gynecol Cancer 25: 961-967, 2015. PMID: 25950130. DOI: 10.1097/IGC.0000000000000463
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top

In this issue

Anticancer Research: 40 (2)
Anticancer Research
Vol. 40, Issue 2
February 2020
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Anticancer Research.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The Significant Association of MMP-1 Genotypes With Taiwan Pterygium
(Your Name) has sent you a message from Anticancer Research
(Your Name) thought you would like to see the Anticancer Research web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
5 + 11 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
The Significant Association of MMP-1 Genotypes With Taiwan Pterygium
CHONG-BIN TSAI, NING-YI HSIA, YUN-CHI WANG, ZHI-HONG WANG, YU-TING CHIN, TAI-LIN HUANG, CHIEN-CHIH YU, WEN-SHIN CHANG, CHIA-WEN TSAI, MEI-CHIN YIN, DA-TIAN BAU
Anticancer Research Feb 2020, 40 (2) 703-707; DOI: 10.21873/anticanres.14000

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
The Significant Association of MMP-1 Genotypes With Taiwan Pterygium
CHONG-BIN TSAI, NING-YI HSIA, YUN-CHI WANG, ZHI-HONG WANG, YU-TING CHIN, TAI-LIN HUANG, CHIEN-CHIH YU, WEN-SHIN CHANG, CHIA-WEN TSAI, MEI-CHIN YIN, DA-TIAN BAU
Anticancer Research Feb 2020, 40 (2) 703-707; DOI: 10.21873/anticanres.14000
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • Association of Matrix Metalloproteinase-1 Genotypes With Bladder Cancer Risk
  • Contribution of Matrix Metalloproteinase-1 Genotypes to Colorectal Cancer in Taiwan
  • The Contribution of MMP-9 Genotypes to Pterygium in Taiwan
  • Significant Association of Interleukin-16 Genetic Variations to Taiwanese Lung Cancer
  • Google Scholar

More in this TOC Section

  • 5-Azacytidine (5-aza) Induces p53-associated Cell Death Through Inhibition of DNA Methyltransferase Activity in Hep3B and HT-29 Cells
  • Prognostic Value of WNT1, NOTCH1, PDGFRβ, and CXCR4 in Oral Squamous Cell Carcinoma
  • Hypoxia-adapted Multiple Myeloma Stem Cells Resist γδ-T-Cell-mediated Killing by Modulating the Mevalonate Pathway
Show more Experimental Studies

Similar Articles

Keywords

  • Age
  • gender
  • Genotype
  • MMP-1
  • polymorphism
  • pterygium
  • Taiwan
Anticancer Research

© 2023 Anticancer Research

Powered by HighWire