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Research ArticleExperimental Studies

A Universal Gelfoam 3-D Histoculture Method to Establish Patient-derived Cancer Cells (3D-PDCC) Without Fibroblasts from Patient-derived Xenografts

JUN YAMAMOTO, NORIHIKO SUGISAWA, KAZUYUKI HAMADA, HIROTO NISHINO, KENTARO MIYAKE, RYUSEI MATSUYAMA, SACHIKO INUBUSHI, HIROKAZU TANINO, MICHAEL BOUVET, ITARU ENDO and ROBERT M. HOFFMAN
Anticancer Research December 2020, 40 (12) 6765-6768; DOI: https://doi.org/10.21873/anticanres.14699
JUN YAMAMOTO
1AntiCancer Inc, San Diego, CA, U.S.A.
2Department of Surgery, University of California, San Diego, La Jolla, CA, U.S.A.;
3Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan
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NORIHIKO SUGISAWA
1AntiCancer Inc, San Diego, CA, U.S.A.
2Department of Surgery, University of California, San Diego, La Jolla, CA, U.S.A.;
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KAZUYUKI HAMADA
1AntiCancer Inc, San Diego, CA, U.S.A.
2Department of Surgery, University of California, San Diego, La Jolla, CA, U.S.A.;
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HIROTO NISHINO
1AntiCancer Inc, San Diego, CA, U.S.A.
2Department of Surgery, University of California, San Diego, La Jolla, CA, U.S.A.;
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KENTARO MIYAKE
3Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan
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RYUSEI MATSUYAMA
3Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan
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SACHIKO INUBUSHI
4Division of Breast and Endocrine Surgery, Kobe University Graduate School of Medicine, Hyogo, Japan
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HIROKAZU TANINO
4Division of Breast and Endocrine Surgery, Kobe University Graduate School of Medicine, Hyogo, Japan
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MICHAEL BOUVET
2Department of Surgery, University of California, San Diego, La Jolla, CA, U.S.A.;
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ITARU ENDO
3Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan
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  • For correspondence: all@anticancer.com endoit@yokohama-cu.ac.jp
ROBERT M. HOFFMAN
1AntiCancer Inc, San Diego, CA, U.S.A.
2Department of Surgery, University of California, San Diego, La Jolla, CA, U.S.A.;
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  • For correspondence: all@anticancer.com endoit@yokohama-cu.ac.jp
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Abstract

Background/Aim: The direct placement of patient tumors in 2-D culture on plastic or glass surfaces has inhibited the establishment of patient-derived cancer cells (PDCCs). The aim of the present study was to develop universal and efficient methods to prepare PDCCs. Materials and Methods: Fragments of patient-derived xenograft (PDX) tumors established form colon cancer liver metastasis (1 mm3) were placed on Gelfoam and cultured in DMEM. Results: PDX tumor fragments were cultured on Gelfoam. Cancer cells migrated from the explant and formed distinct 3-D structures in the Gelfoam. Each of the three PDCCs showed a distinct morphology. The cultures were essentially all cancer cells without fibroblasts, the opposite of what usually occurs in 2-D culture on plastic or glass. Gelfoam cultures could be readily passaged from one Gelfoam cube to anothers suggesting indefinite culture potential. Conclusion: A potentially universal method to establish PDCC using PDX tumors and 3-D Gelfoam histoculture was developed.

Key Words:
  • 3-D culture
  • histoculture
  • Gelfoam
  • patient-derived cancer cells
  • PDCC
  • patient-derived xenograft
  • PDX
  • Received October 30, 2020.
  • Revision received November 16, 2020.
  • Accepted November 17, 2020.
  • Copyright © 2020 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 40 (12)
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A Universal Gelfoam 3-D Histoculture Method to Establish Patient-derived Cancer Cells (3D-PDCC) Without Fibroblasts from Patient-derived Xenografts
JUN YAMAMOTO, NORIHIKO SUGISAWA, KAZUYUKI HAMADA, HIROTO NISHINO, KENTARO MIYAKE, RYUSEI MATSUYAMA, SACHIKO INUBUSHI, HIROKAZU TANINO, MICHAEL BOUVET, ITARU ENDO, ROBERT M. HOFFMAN
Anticancer Research Dec 2020, 40 (12) 6765-6768; DOI: 10.21873/anticanres.14699

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A Universal Gelfoam 3-D Histoculture Method to Establish Patient-derived Cancer Cells (3D-PDCC) Without Fibroblasts from Patient-derived Xenografts
JUN YAMAMOTO, NORIHIKO SUGISAWA, KAZUYUKI HAMADA, HIROTO NISHINO, KENTARO MIYAKE, RYUSEI MATSUYAMA, SACHIKO INUBUSHI, HIROKAZU TANINO, MICHAEL BOUVET, ITARU ENDO, ROBERT M. HOFFMAN
Anticancer Research Dec 2020, 40 (12) 6765-6768; DOI: 10.21873/anticanres.14699
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Keywords

  • 3-D culture
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