Pollen Allergy Suppression Effect by the Oral Administration of Acetic Acid Bacteria (Gluconacetobacter hansenii)

HIROYUKI INAGAWA; TAKASHI NISHIZAWA; CHIE KOCHI; SATOKO AMANO; GEN-ICHIRO SOMA

doi:10.21873/anticanres.13627

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Figure 1.
Macrophage activation effect of various G. hansenii cell preparation samples. Each sample was added to RAW 246.7 cells, and after 20 h, the amount of NO₂ in the supernatant was measured using the Griess reaction. ○: Positive control (Pantoea agglomerans LPS), ●: Freeze-dried G. hansenii viable cells, ▪: G. hansenii viable cells (pH 4 to 5) freeze-dried after heat-killing, □: G. hansenii viable cells heat-killed and freeze-dried after neutralization (pH 7).
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Figure 2.
Effect of oral administration of the prepared G. hansenii cell samples on cedar pollen-immunized mice. After 3 intraperitoneal administrations of cedar pollen and alum to mice, cedar pollen was administered intranasally for 2 weeks, and total IgE levels in blood were evaluated (day 28). Killed G. hansenii (0, 30, 150 mg/kg) were administered orally with a feeding needle and cedar pollen was administered intranasally for up to 2 weeks (day 30 to 44). On day 44, the frequency of nose scratching following the cedar pollen immunization was counted for 30 min. Blood was collected, and the total IgE levels in serum were evaluated. A: Total IgE levels on day 28 of the non-immunized and immunized control groups (killed G. hansenii 0, 30, 150 mg/kg). B: Body weight of the test groups on day 44. C: Frequency of nose scratching over 30 minutes on day 44. D: Total IgE levels in blood on day 44. Columns and bars indicate means and standard deviations (n=9-16), respectively. Different letters indicate statistically significant differences between groups (ANOVA, p<0.05).
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Figure 3.
Cytokine analysis of cedar pollen-immunized mice orally administered with the prepared samples of G. hansenii cells. After 3 intraperitoneal administrations of cedar pollen and alum to mice, cedar pollen was administered intranasally for 2 weeks, and then killed G. hansenii (0, 30, 150 mg/kg) were administered orally with a feeding needle for up to 4 weeks (day 30 to 58) and cedar pollen was administered intranasally for up to 3 weeks (day 37 to 58). On day 58, the collection of blood samples and extraction of Peyer's patches and spleen was performed. A: Serum IL-10 was measured using ELISA. Different letters indicate statistically significant differences between groups (ANOVA, p<0.01). B: RNA was extracted from Peyer's patches, and quantitative RT-PCR was performed. The vertical axis shows the relative amounts of gene expression for the immunized control group (b), immunized + acetic acid bacteria low dosage group (c), and immunized + acetic acid bacteria high dosage group (d) when gene-expression in the non-immunized control group (a) is considered as 1. The column shows the mean, and the bar shows the SD (n=9 to 16). C: Gene expression of cytokines in the spleen.