Figure 4. Cytokine analysis of the CM from stromal cells. (A) Profiles of cytokines in the CM from cultures of stromal cells. Antibodies to 102 cytokines were spotted on nitrocellulose membranes. Angiopoietin-2 was found at higher levels in the CM from oligodendrocytes, ODC1 and ODC2, compared to that from fibroblasts, GF1 (arrow). In contrast, chitinase 3-like 1, DPPIV, CXCL5, GM-CSF, IGFBP-3, IL-6, IL-8, IP-10, kallikrein 3, thrombospondin-1, and VEGF, were produced at higher levels from fibroblasts, compared to oligodendrocytes. (B) Production of angiopoietin-2 by oligodendrocytes and fibroblasts was assayed by ELISA. Oligodendrocytes, ODC1 and ODC2, produced more than 5 ng/ml of angiopoietin-2. Fibroblasts, GF1, produced lower levels of angiopoietin-2.