Article Figures & Data
Figures
- Figure 1.
Synthesis of bis(4-hydroxy-2H-chromen-2-one) coumarin (4HC).
- Figure 2.
Proliferation of MCF-10a mammary non-cancerous cells (A) and MCF-7 mammary cancer cells (B) treated with 4HC. 4HC showed significant anti-proliferative activities in MCF-7 cells in time and concentration-dependent manner. *p≤0.05; **p≤0.01; ***p≤0.001 compared to the control value. Experiments were repeated in triplicate. Ctl, Control; Doxo, doxorubicin.
- Figure 3.
MCF-7 cell cycle distribution analysis (A). The percentage of cells in the G1, S, and G2/M phases of the cell cycle are shown, following a treatment with 25 and 50 μM of 4HC for 72 h (B). *p≤0.05; **p≤0.01 compared to the control group. Experiments were repeated in triplicate. PI, Propidium iodide; ctl, control.
- Figure 4.
Evaluation of apoptosis in MCF-7 cells treated with 4HC. Images (captured by FCS express 6 image Cytometry, De Novo software) showed apoptosis in MCF-7 cells. Control and 4HC-treated cells for 72 h were stained with Annexin V-FITC and PI. (FITC-/PI-) reflected living cells, fluorescence (FITC+/PI-) represented early apoptotic cells, and fluorescence (FITC+/PI+) showed late apoptotic cells (A). The percentage of apoptosis is represented as the mean±SD (B). *p≤0.05; **p≤0.01, compared to the control. Experiments were repeated in triplicate. Ctl, Control.
- Figure 5.
The relative expression of the pro-apoptotic Bax, and caspase 3 genes, the anti-apoptotic gene Bcl-2 and the aromatase gene CYP19A1 levels in 4HC-treated and control MCF-7 cells (for 72 h) were analyzed using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). *p≤0.05; **p≤0.01, compared to the control group. Experiments were repeated in triplicate. Ctl, Control.
