Figure 5. Etomidate affected the level of proteins associated with migration and invasion of A549 cells. Cells (1×106 cells/well) were treated with 0.2, 0.4 and 0.8 μg/ml of etomidate for 24 and 48 h. Total proteins were collected and the levels of A: RAS, phospho-jun proto-oncogene (p-c-JUN), protein tyrosine kinase 2 (p-FAK), N-cadherin, AKT serine/threonine kinase 1 (p-AKTThr308); B: E-cadherin, ras homolog family member A (RHOA), matrix metallopeptidase 10 (MMP10), protein kinase C (PKC), MMP7; C: phosphoinositide 3-kinase (PI3K), snail family transcriptional repressor 1 (SNAI1), MMP1, MMP9, SOS RAS/RAC guanine nucleotide exchange factor 1 (SOS1); D: p-p38, MMP2, plasminogen activator, urokinase (uPA); and E: phospho-extracellular signal-regulated protein kinases 1 and 2 (pERK1/2), growth factor receptor bound protein 2 (GRB2), β-catenin, TIMP metallopeptidase inhibitor 1 (TIMP1) and phospho-c-Jun N-terminal kinases (p-JNK1/2) were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting as described in the Materials and Methods. Direct re-probing with β-actin antibody was used as an internal control.