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Research ArticleClinical Studies

Pilot Study of Probe-based Confocal Laser Endomicroscopy with Fluorescein-dripping Method for Liver Tumors

MASAKI MORIMOTO, SOICHIRO HONJO, TERUHISA SAKAMOTO, EI UCHINAKA, MASATAKA AMISAKI, YOSUKE ARAI, MANABU YAMAMOTO, YOJI FUKUMOTO, TOMOHIRO OSAKI, NARUO TOKUYASU, KEIGO ASHIDA, HIROAKI SAITO and YOSHIYUKI FUJIWARA
Anticancer Research August 2018, 38 (8) 4775-4781; DOI: https://doi.org/10.21873/anticanres.12786
MASAKI MORIMOTO
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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SOICHIRO HONJO
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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  • For correspondence: honjo{at}tottori-u.ac.jp
TERUHISA SAKAMOTO
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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EI UCHINAKA
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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MASATAKA AMISAKI
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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YOSUKE ARAI
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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MANABU YAMAMOTO
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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YOJI FUKUMOTO
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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TOMOHIRO OSAKI
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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NARUO TOKUYASU
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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KEIGO ASHIDA
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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HIROAKI SAITO
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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YOSHIYUKI FUJIWARA
Department of Surgery, Division of Surgical Oncology, Faculty of Medicine, Tottori University, Yonago, Japan
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    Figure 1.

    Fluorescein dripping and pCLE evaluation after washing with normal saline.

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    Figure 2.

    Observation of representative images at three exposure times. Scale bar=50 μm.

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    Figure 3.

    pCLE images with fluorescein dripping in HCC cancerous regions and the corresponding pathological examination findings. Scale bar=50 μm.

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    Figure 4.

    Other tumors except for HCC. Scale bar=50 μm.

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    Figure 5.

    The noncancerous region showed a regular arrangement of hepatocytes with fluorescein dripping. Four representative cases. Scale bar=50 μm.

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    Figure 6.

    Another demo-probe similar to the GastroFlex probe shows HCC tumor cells with high magnification. Scale bar=20 μm.

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Anticancer Research
Vol. 38, Issue 8
August 2018
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Pilot Study of Probe-based Confocal Laser Endomicroscopy with Fluorescein-dripping Method for Liver Tumors
MASAKI MORIMOTO, SOICHIRO HONJO, TERUHISA SAKAMOTO, EI UCHINAKA, MASATAKA AMISAKI, YOSUKE ARAI, MANABU YAMAMOTO, YOJI FUKUMOTO, TOMOHIRO OSAKI, NARUO TOKUYASU, KEIGO ASHIDA, HIROAKI SAITO, YOSHIYUKI FUJIWARA
Anticancer Research Aug 2018, 38 (8) 4775-4781; DOI: 10.21873/anticanres.12786

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Pilot Study of Probe-based Confocal Laser Endomicroscopy with Fluorescein-dripping Method for Liver Tumors
MASAKI MORIMOTO, SOICHIRO HONJO, TERUHISA SAKAMOTO, EI UCHINAKA, MASATAKA AMISAKI, YOSUKE ARAI, MANABU YAMAMOTO, YOJI FUKUMOTO, TOMOHIRO OSAKI, NARUO TOKUYASU, KEIGO ASHIDA, HIROAKI SAITO, YOSHIYUKI FUJIWARA
Anticancer Research Aug 2018, 38 (8) 4775-4781; DOI: 10.21873/anticanres.12786
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Keywords

  • Hepatocellular carcinoma
  • liver tumor
  • confocal laser endomicroscopy
  • fluorescein
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