Abstract
Background: The aim of this study was to analyze the spatial distribution and proliferation of adoptively transferred CD8+ T-lymphocytes sensitized against allogeneic tumors. Materials and Methods: Transgenic β-actin-luc mice that express luciferase were sensitized against allogeneic SL2 lymphoma. CD8+ T-lymphocytes from these mice were transferred to lymphocyte-deficient, recombination activating gene-deficient (Rag−/−) mice bearing SL2 tumors and were tracked using bioluminescence imaging. Results: Two out of six Rag−/− mice rejected their tumors. There were no apparent differences in spatial distribution and proliferative intensity of adoptively-transferred CD8+ T-lymphocytes between the two Rag−/− mice that rejected allogeneic SL2 tumors and the four Rag−/− mice that did not. Conclusion: The pattern of distribution in the mouse body and proliferative intensity of CD8+ T-lymphocytes do not seem to be decisive factors influencing allogeneic tumor rejection.
- Received June 19, 2018.
- Revision received June 28, 2018.
- Accepted June 29, 2018.
- Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved