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Research ArticlePROCEEDINGS OF THE 21st ANNUAL MEETING OF THE SOCIETY OF BIOTHERAPEUTIC APPROACHES

Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy

YUKI ABE, HIROHITO KOBAYASHI, YOSHIKA AKIZAWA, KEN ISHITANI, KAZUNORI HASHIMOTO and HIDEO MATSUI
Anticancer Research July 2018, 38 (7) 4327-4331; DOI: https://doi.org/10.21873/anticanres.12732
YUKI ABE
1Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan
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HIROHITO KOBAYASHI
2Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan
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YOSHIKA AKIZAWA
1Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan
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KEN ISHITANI
3Department of Gynecology, Kitasato University Kitasato Institute Hospital, Tokyo, Japan
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KAZUNORI HASHIMOTO
1Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan
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  • For correspondence: hashimoto.kazunori@twmu.ac.jp
HIDEO MATSUI
1Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan
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    Figure 1.

    Ratio Vδ2+ T cells/CD3+ cell and proliferation of ratio Vδ2+ T cells in peripheral blood and ascites. The proportion of Vδ2+ T cells in CD3+ cells in peripheral blood from 20 patients with ovarian cancer, one patient with fallopian tube cancer; and six patients with primary peritoneal cancer and from the ascites of 11 patients with ovarian cancer and five patients with primary peritoneal cancer (A). The proportion of Vδ2+ T cells relative to CD3+ cells after culture with each antigen (B). Proliferation of Vδ2+ T cells from blood and ascites after 14 days in culture with 2M3B1-PP or Zol (C).

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    Figure 2.

    Representative data from one patient with stage 3c primary peritoneal cancer. Cells were either pre-cultured (a) or cultured in 2M3B1-PP+IL-2 (b) or Zol+ IL-2 (c) for 14 days. PBMCs and tumour cells from ascites were stained with anti-CD3, anti-CD56, and anti-Vδ2 antibodies and analysed using flow cytometry (A). Dot plots of CD56- and Vδ2-stained and gated CD3+ T cells are shown. In addition, absolute numbers of Vδ2+ (B) and tumour (C) cells were determined.

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Anticancer Research: 38 (7)
Anticancer Research
Vol. 38, Issue 7
July 2018
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Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy
YUKI ABE, HIROHITO KOBAYASHI, YOSHIKA AKIZAWA, KEN ISHITANI, KAZUNORI HASHIMOTO, HIDEO MATSUI
Anticancer Research Jul 2018, 38 (7) 4327-4331; DOI: 10.21873/anticanres.12732

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Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy
YUKI ABE, HIROHITO KOBAYASHI, YOSHIKA AKIZAWA, KEN ISHITANI, KAZUNORI HASHIMOTO, HIDEO MATSUI
Anticancer Research Jul 2018, 38 (7) 4327-4331; DOI: 10.21873/anticanres.12732
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Keywords

  • γδ T cells
  • ovarian cancer
  • ascites
  • tumour-infiltrating lymphocytes
  • ascites-infiltrating lymphocytes
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