Article Figures & Data
Figures
- Figure 1.
Recombinant targeting modules (scFvs) prepared using a bacterial expression system. A. Lower, Map of expression vector for scFvs (pscFv_HL/ pscFv_LH). Upper, Schematic diagram of recombinant scFvs possessing SrtA recognition sequence LPETGG followed by His-tag for Ni-affinity purification. B: SDS-PAGE image (CBB staining) of the purified recombinant scFvs. Both scFv_HL and scFv_LH (indicated by arrowhead) were prepared with high purity and the calculated molecular weight of each antibody was observed to be 27.9 kDa.
- Figure 2.
Recombinant targeting module (Fab) prepared using a mammalian cell expression system. A. Lower, Map of expression vector for Fab (pOTC-tHChg9/pOTC-tLC). Only pOTC-tHChg9 has the sequence for LPETGG at the downstream of the sequence for hinge region. Upper, Schematic diagram of recombinant Fab possessing SrtA recognition sequence LPETGG at the C-terminal of heavy chain fragment, which is connected with light chain by disulfide bond. B: SDS-PAGE image (CBB staining) of the purified recombinant Fab. Fab (indicated by arrowhead) was prepared with high purity and the calculated molecular weight was observed to be about 45.0 kDa, which is lower than its calculated molecular weight (53.8 kDa).
- Figure 3.
Transcription of the HER2 gene in human cell lines. Transcription of the gene encoding HER2 was investigated in HCT-15 and HeLa cell lines. The gene encoding GAPDH was used as an internal standard for the assay. This experiment was conducted twice, and one of the results is shown.
- Figure 4.
Reactivity of the anti-HER2 recombinant fragment antibodies against HER2-positive cells. HCT-15 and HeLa cells were treated with anti-HER2 recombinant fragment antibodies as primary antibodies and Alexa Fluor 488-labeled secondary antibodies. The fluorescent image of Alexa Fluor 488 was observed using the IX71 fluorescence microscope (Olympus, Tokyo, Japan). BG: Samples prepared without anti-HER2 recombinant treatment. Scale bars indicate 10 μm.
