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Research ArticlePROCEEDINGS OF THE 21st ANNUAL MEETING OF THE SOCIETY OF BIOTHERAPEUTIC APPROACHES

Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity

RIHO MASHIBA, MAMI ISHIKAWA, YU SUMIYA, KUMPEI KAWAKATSU, NGOC KIET TRAN and TAKAHITO NISHIKATA
Anticancer Research July 2018, 38 (7) 4295-4298; DOI: https://doi.org/10.21873/anticanres.12727
RIHO MASHIBA
1Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan
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MAMI ISHIKAWA
1Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan
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YU SUMIYA
1Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan
2Club Cosmetics Co. Ltd, Osaka, Japan
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KUMPEI KAWAKATSU
1Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan
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NGOC KIET TRAN
1Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan
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TAKAHITO NISHIKATA
1Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan
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  • For correspondence: nisikata@konan-u.ac.jp
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    Figure 1.

    Difference in phagocytic activity of THP-1 macrophages treated with MAFs. Phagocytic activity was evaluated by using fluorescence (IBRf) to determine the internalised bead ratio. Details of these calculations are described in the Materials and Methods section. MAFs used in this experiment are as follows: 1 μg/ml LPS + 1 ng/ml IFN-γ, 8 μg/ml serum MAF. Error bars represent SDs (n=3). *p=0.0003 (t-test).

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    Figure 2.

    Migratory activity of THP-1 macrophages treated with MAFs. The average migration distance of macrophages was analyzed (A). Error bars represent SD (n=3). *p=0.40, and **p=0.02 (t-test). Plots of migration distance versus the number of engulfed beads of each macrophage are shown (B-D). MAFs used in this experiment are as follows: no MAFs (B), 1 μg/ml LPS + 1 ng/ml IFN-γ (C), 8 μg/ml serum MAF (D).

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    Figure 3.

    Phagocytic assay treated with MAFs in suspension conditions. MAFs used in this experiment are as follows: 1 μg/ml LPS + 1 ng/ml IFN-γ, 8 μg/ml serum MAF. Error bars represent SD (n=3). *p=0.0004 (t-test).

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Anticancer Research: 38 (7)
Anticancer Research
Vol. 38, Issue 7
July 2018
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Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity
RIHO MASHIBA, MAMI ISHIKAWA, YU SUMIYA, KUMPEI KAWAKATSU, NGOC KIET TRAN, TAKAHITO NISHIKATA
Anticancer Research Jul 2018, 38 (7) 4295-4298; DOI: 10.21873/anticanres.12727

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Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity
RIHO MASHIBA, MAMI ISHIKAWA, YU SUMIYA, KUMPEI KAWAKATSU, NGOC KIET TRAN, TAKAHITO NISHIKATA
Anticancer Research Jul 2018, 38 (7) 4295-4298; DOI: 10.21873/anticanres.12727
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Keywords

  • THP-1
  • serum MAF (serum-derived macrophage activating factor)
  • pH-sensitive chromophore
  • suspension assay
  • time-lapse image analysis
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