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Research ArticlePROCEEDINGS OF THE 21st ANNUAL MEETING OF THE SOCIETY OF BIOTHERAPEUTIC APPROACHES

Evaluation of a Hypertensive Rat Model Using Peripheral Blood Neutrophil Activity, Phagocytic Activity and Oxidized LDL Evaluation

RAN ZHANG, HIROYUKI INAGAWA, KIMIKO KAZUMURA, HIROSHI TSUCHIYA, TOSHIYUKI MIWA, NAOKAZU MORISHITA, SACHIKO UCHIBORI, JUN HANASHIRO, TSUTOMU MASAKI, HIDEKI KOBARA and GEN-ICHIRO SOMA
Anticancer Research July 2018, 38 (7) 4289-4294; DOI: https://doi.org/10.21873/anticanres.12726
RAN ZHANG
1Control of Innate Immunity, Technology Research Association, Kagawa, Japan
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  • For correspondence: zhang@shizenmeneki.org
HIROYUKI INAGAWA
1Control of Innate Immunity, Technology Research Association, Kagawa, Japan
2Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan
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KIMIKO KAZUMURA
3Central Research Laboratory, Hamamatsu Photonics K.K., Hamamatsu, Japan
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HIROSHI TSUCHIYA
3Central Research Laboratory, Hamamatsu Photonics K.K., Hamamatsu, Japan
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TOSHIYUKI MIWA
3Central Research Laboratory, Hamamatsu Photonics K.K., Hamamatsu, Japan
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NAOKAZU MORISHITA
4Electron Tube Division, Hamamatsu Photonics K.K., Hamamatsu, Japan
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SACHIKO UCHIBORI
1Control of Innate Immunity, Technology Research Association, Kagawa, Japan
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JUN HANASHIRO
1Control of Innate Immunity, Technology Research Association, Kagawa, Japan
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TSUTOMU MASAKI
5Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Kagawa, Japan
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HIDEKI KOBARA
5Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Kagawa, Japan
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GEN-ICHIRO SOMA
1Control of Innate Immunity, Technology Research Association, Kagawa, Japan
2Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan
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    Figure 1.

    Changes in blood pressure over time between the SHR group and WKY group. Systolic blood pressure (SBP) (A), diastolic blood pressure (DBP) (B), and mean blood pressure (MBP) (C) were measured at 6 weeks old, 10 weeks old, 13 weeks old and 16 weeks old rats. Black circle: WKY group; black square: SHR group. The data are presented as the means±SEM and were obtained from 3 rats per group. *p<0.05, **p<0.01, ***p<0.001 compared to the WKY group.

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    Figure 2.

    The level of plasma biochemical markers between the SHR and WKY group. LDL-C: Low density lipoprotein cholesterol (A), HDL-C: high density lipoprotein cholesterol (B), T-CHO: total cholesterol (C), TG: triglyceride (D), ALT: alanine aminotransferase (E), T-BIL: total bilirubin (F). Black column: WKY group; gray column: SHR group. The data are presented as the means±SEM and were obtained from 3 rats per group. **p<0.01, ***p<0.001 compared to the WKY group.

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    Figure 3.

    Changes in O2•−, MPO, PFI, and OxLDL. O2− changes at 6 weeks old and 16 weeks old rats (A), MPO changes in at 6 weeks old and 16 weeks old rats (B), PFI changes at 6 weeks old and 16 weeks old rats (C). Black circle: WKY group; black square: SHR group. OxLDL was measured at 16 weeks old rats (D). Black column: WKY group; gray column: SHR group. The data are presented as the means±SEM and were obtained from 3 rats per group. **p<0.01, ***p<0.001 compared to the WKY group.

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Anticancer Research: 38 (7)
Anticancer Research
Vol. 38, Issue 7
July 2018
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Evaluation of a Hypertensive Rat Model Using Peripheral Blood Neutrophil Activity, Phagocytic Activity and Oxidized LDL Evaluation
RAN ZHANG, HIROYUKI INAGAWA, KIMIKO KAZUMURA, HIROSHI TSUCHIYA, TOSHIYUKI MIWA, NAOKAZU MORISHITA, SACHIKO UCHIBORI, JUN HANASHIRO, TSUTOMU MASAKI, HIDEKI KOBARA, GEN-ICHIRO SOMA
Anticancer Research Jul 2018, 38 (7) 4289-4294; DOI: 10.21873/anticanres.12726

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Evaluation of a Hypertensive Rat Model Using Peripheral Blood Neutrophil Activity, Phagocytic Activity and Oxidized LDL Evaluation
RAN ZHANG, HIROYUKI INAGAWA, KIMIKO KAZUMURA, HIROSHI TSUCHIYA, TOSHIYUKI MIWA, NAOKAZU MORISHITA, SACHIKO UCHIBORI, JUN HANASHIRO, TSUTOMU MASAKI, HIDEKI KOBARA, GEN-ICHIRO SOMA
Anticancer Research Jul 2018, 38 (7) 4289-4294; DOI: 10.21873/anticanres.12726
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Keywords

  • Peripheral blood
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  • phagocytic activity
  • oxidized LDL
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