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Research ArticleExperimental Studies

Demethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-κB Pathways

CHIN-CHUNG LIN, CHAO-LIN KUO, YI-PING HUANG, CHENG-YEN CHEN, MING-JIE HSU, YUNG LIN CHU, FU-SHIN CHUEH and JING-GUNG CHUNG
Anticancer Research May 2018, 38 (5) 2761-2769;
CHIN-CHUNG LIN
1Departments of Chinese Medicine, Feng-Yuan Hospital, Ministry of Health and Welfare, Executive Yuan, Taichung, Taiwan, R.O.C.
2General Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C.
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CHAO-LIN KUO
3Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C.
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YI-PING HUANG
4Department of Physiology, China Medical University, Taichung, Taiwan, R.O.C.
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CHENG-YEN CHEN
5Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
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MING-JIE HSU
5Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
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YUNG LIN CHU
6International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C.
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FU-SHIN CHUEH
7Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
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  • For correspondence: jgchung@mail.cmu.edu.tw fushin@asia.edu.tw
JING-GUNG CHUNG
5Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
8Department of Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
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  • For correspondence: jgchung@mail.cmu.edu.tw fushin@asia.edu.tw
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Abstract

Background/Aim: Demethoxycurcumin (DMC), one of the curcuminoids present in turmeric, has been shown to induce cell death in many human cancer cell lines, however, there has not been any investigation on whether DMC inhibits metastatic activity in human cervical cancer cells in vitro. In the present study, DMC at 2.5-15 μM decreased cell number, thus, we used IC20 (7.5 μM) for further investigation of its anti-metastatic activity in human cervical cancer HeLa cells. Materials and Methods: The wound healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of DMC on HeLa cells. Results: The wound healing assay was used to show that DMC suppressed cell movement of HeLa cells. Furthermore, the trans-well chamber assay was used to show that DMC suppressed HeLa cell migration and invasion. Gelatin zymography assay did not show any significant effects of DMC on the gelatinolytic activity (MMP-2 and -9) in conditioned media of HeLa cells treated by DMC. Western blotting showed that DMC significantly reduced protein levels of GRB2, MMP-2, ERK1/2, N-cadherin and Ras but increased the levels of E-cadherin and NF-κB in HeLa cells. Confocal laser microscopy indicated that DMC increased NF-κB in HeLa cells confirming the results from Western blotting. Conclusion: DMC may be used as a novel anti-metastatic agent for the treatment of human cervical cancer in the future.

  • Demethoxycurcumin (DMC)
  • migration
  • invasion
  • NF-κB
  • p65
  • HeLa cells
  • Received March 19, 2018.
  • Revision received April 3, 2018.
  • Accepted April 10, 2018.
  • Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
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Anticancer Research: 38 (5)
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Demethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-κB Pathways
CHIN-CHUNG LIN, CHAO-LIN KUO, YI-PING HUANG, CHENG-YEN CHEN, MING-JIE HSU, YUNG LIN CHU, FU-SHIN CHUEH, JING-GUNG CHUNG
Anticancer Research May 2018, 38 (5) 2761-2769;

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Demethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-κB Pathways
CHIN-CHUNG LIN, CHAO-LIN KUO, YI-PING HUANG, CHENG-YEN CHEN, MING-JIE HSU, YUNG LIN CHU, FU-SHIN CHUEH, JING-GUNG CHUNG
Anticancer Research May 2018, 38 (5) 2761-2769;
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Keywords

  • Demethoxycurcumin (DMC)
  • migration
  • invasion
  • NF-κB
  • p65
  • HeLa cells
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