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Research ArticleExperimental Studies

Antitumor Activity of Novel Bone-seeking, α-emitting 224Ra-solution in a Breast Cancer Skeletal Metastases Model

ASTA JUZENIENE, JENNI BERNOULLI, MARI SUOMINEN, JUSSI HALLEEN and ROY HARTVIG LARSEN
Anticancer Research April 2018, 38 (4) 1947-1955;
ASTA JUZENIENE
1Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
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JENNI BERNOULLI
2Pharmatest Services Ltd, Turku, Finland
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MARI SUOMINEN
2Pharmatest Services Ltd, Turku, Finland
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JUSSI HALLEEN
2Pharmatest Services Ltd, Turku, Finland
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ROY HARTVIG LARSEN
3Sciencons AS, Oslo, Norway
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  • For correspondence: royhlars1@gmail.com
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Abstract

Background/Aim: Bone metastases are associated with increased morbidity and poor prognosis in a variety of cancers. The present study investigated the effects of targeted radionuclide therapy with α-emitting, bone-seeking radium-224 (224Ra) on osteolytic bone metastasis of MDA-MB-231(SA)-GFP human breast cancer cells injected intracardially into nude mice. Materials and Methods: Vehicle, ethylenediamine tetra (methylene phosphonic acid) (EDTMP) and 224Ra-solution (45, 91 or 179 kBq/kg) with EDTMP were intravenously administered to mice two days after cell injection. The bone-seeking EDTMP was added to the 224Ra-solution to improve bone targeting of 212Pb, which is a progeny of 224Ra. Results: Radium-224 solution treatment decreased in a dose-dependent manner the areas of osteolytic lesions in the hind limbs and the number of tumor foci in the whole skeleton, and extended survival. Paraplegia was not observed in 179 kBq/kg 224Ra-solution group. Conclusion: Radium-224-solution containing chelated 212Pb is a promising candidate for the treatment of breast cancer patients with bone metastases.

  • Bone metastasis
  • breast cancer
  • radionuclide
  • radium-224
  • targeted α-therapy

Bone is the most frequent site of metastasis among patients with advanced breast and prostate cancer (1). Bone metastases lead to pain and skeletal-related events (pathological fractures, spinal cord compression, hypercalcemia of malignancy), which cause significant morbidity and reduced quality of life (1). The development of bone metastases is associated with shortened life expectancy for patients (1). Available treatments such as ablation, bisphosphonates, radiotherapy, chemotherapy, monoclonal antibody therapy often show limited benefits (1). Prevention and effective treatment of bone metastases can have a significant positive impact on the outcomes of patients with advanced cancers.

Targeted radionuclide therapies based on β-emitting radioisotopes have long been used to alleviate pain in patients with bone metastases from breast and prostate cancer (1). However, β-emitting radionuclides can induce myelosuppression (1). Radium-223 (223Ra) dichloride (Xofigo®, Bayer HealthCare Pharmaceuticals Inc.) is the first-in-class commercially available α-emitter approved by the FDA and EMA in 2013 for the treatment of bone metastases in patients with metastatic castrate-resistant prostate cancer. This treatment improves overall survival and delays symptomatic skeletal events (2). Radium-223, as a calcium mimetic, has a natural bone-seeking capability and preferentially binds to areas of increased bone turnover in bone metastases targeting osteoblastic metastatic lesions (2). The efficacy of 223Ra has been demonstrated in animal models indicating significant antitumor effect in experimental skeletal metastases in nude rats (3), increased survival in a mouse model of breast (4) and antitumor effect in prostate cancer growth in mouse bone (5). Toxic effects on adjacent tissues and particularly the bone marrow are minimal due to a short path length (<100 μm) of the α-particles.

Similarly to 223Ra, 224Ra is a natural bone-seeking α-emitting radionuclide, and its bone-seeking properties in humans are well known (6). The attractive aspect of 224Ra is the shorter half-life (3.63 days) than that of 223Ra (11.43 days). Radium-224 solutions have been used earlier in the treatment of noncancerous bone diseases such as ankylosing spondylitis (6-8). There are limited reports on the development of radiopharmaceuticals or devices with 224Ra for cancer treatment (9, 10). Preclinical results in mice have demonstrated that 224Ra loaded wires (7-65 kBq) inserted into solid tumors can effectively destroy tumors (9, 10).

One potential problem with 224Ra solutions is the generation of the progeny β-emitting lead-212 (212Pb) with the significant half-life of 10.6 h (Figure 1). Lead-212 together with the α-emitting progeny bismuth-212 (212Bi) could contribute to unwanted non-target tissue and cell exposure (11, 12). However, 212Pb and 212Bi have been successfully used with different carriers in preclinical and clinical studies (12-15). The bone-seeking ethylenediamine tetra (methylene phosphonic acid) (EDTMP) was suggested to direct 212Pb to bone (11, 16). Recently, Larsen demonstrated that the addition of EDTMP to 224Ra solution reduced the uptake of 212Pb in soft tissues compared with 224Ra without EDTMP (16). In the current study EDTMP was added to reduce soft tissue exposure to 212Pb and to exploit its daughter α-emitting 212Bi as part of the therapy. This was achieved by in situ generation of the bone-seeking 212Pb-EDTMP complex in the 224Ra-solution, thus, increasing bone uptake and soft tissue elimination of 212Pb (16). At secular equilibrium, the number of 212Pb atoms is about 14% of the 224Ra atoms (16). It is therefore advantageous to complex 212Pb before injection of 224Ra solution which may be stored for a while as it would be the case with centralized production and long-distance shipment of 224Ra-solutions.

In this work, we studied the effects of a single injection of 224Ra solution with 212Pb-EDTMP in a mouse model mimicking breast cancer skeletal metastasis. This solution containing 224Ra in equilibrium with 212Pb-EDTMP is hereafter denoted as the 224Ra-solution.

Materials and Methods

Cell culture. Human MDA-MB-231(SA) breast cancer cells were kindly provided by Dr. T. Guise (Indiana, USA) (17). These cells were transfected with green fluorescent protein (GFP, pTurboGFP-N vector, Evrogen JSC, clone 6, Moscow, Russia). MDA-MB-231(SA)-GFP cells were authenticated in June 2014 using short tandem repeat analysis (GenePrint10 system, Promega, Madison, WI, USA) at the Finland Institute for Molecular Medicine (FIMM, Helsinki, Finland). The authenticated frozen stock was used in the study. MDA-MB-231(SA)-GFP cells were grown in DMEM media supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 1% non-essential amino acids, 100 U/ml penicillin and 100 μg/ml streptomycin in an incubator with a humidified atmosphere of 95% air and 5% CO2 at 37°C.

Preparation of the 224Ra solution. Radium-224 was extracted from a generator based on 228Th (Eckert & Ziegler, Braunschweig, Germany) immobilized on a DIPEX® (Eichrom Technologies LLC, Lisle, IL, USA) actinide resin (18). It was purified and found with no traces of 228Th (18). The details of the 224Ra generator setup have been described elsewhere. Radium-224 (half-life 3.66 days) was in equilibrium with daughter radionuclides including 212Pb (half-life 10.6 h). Radium-224 was combined with EDTMP to complex free 212Pb in the solution to make 212Pb a better bone seeker and to avoid its uptake in hematopoietic cells etc. (16). A concentration of 5 mg/ml EDTMP was used.

Radioactivity measurements. Since 224Ra decay has a modest γ-emission in an energy region with more abundant γ from daughter 212Pb, 224Ra activity was determined indirectly from the counts in the 70-80 keV or 60-110 keV windows. The details of the radioactivity measurements have been described elsewhere (18). A Capintec CRC-25R dose calibrator and Cobra II Autogamma counter (Packard Instrument, Downers Grove, II, USA) were used for radioactivity measurements.

Animals. Female nude mice (Hsd:Athymic nude-Foxn1nu, Harlan Laboratories B.V., currently Envigo, Horst, the Netherlands) were used. The mice were 4-5 weeks of age weighing approximately 20 g at the beginning of the study. These animal studies were approved by the National Committee for Animal experiments of Finland. All experiments were performed in accordance with relevant guidelines and regulations. Mice were kept under pathogen-free and controlled conditions and fed with a soy-free diet (Harlan Teklad Global Rodent diets, irradiated 2916, Harlan Laboratories, B.V., currently Envigo, Horst, the Netherlands) and tap water ad libitum. The mice were randomized based on body weight into five groups (12 animals per group) and marked with ear marks.

On day 0, MDA-MB-231(SA)-GFP cells (105 in 0.1 ml PBS) were inoculated into the left cardiac ventricle of mice. Analgesia (0.1 mg/kg buprenorphine administered subcutaneously) was given at least one hour before intracardiac inoculation, which was performed under isoflurane anesthesia. Viability of cells was determined before and after inoculations.

On day 2, mice received either intravenous injections (5 μl per g body weight) of (1) vehicle (isotonic sodium chloride, pH 5-7), (2) bone-seeking EDTMP (ethylenediamine tetra (methylene phosphonic acid)), 5 mg/ml in sodium chloride, (3) 45 kBq, 91 kBq, or 179 kBq 224Ra-solutions (supplied with 5 mg/ml EDTMP).

Mice were given analgesia (0.02 mg/ml buprenorphine in the drinking water) during the last study days when needed.

Radiography and fluorescence imaging were performed at the end of the study (at sacrifice) to detect metastatic lesions.

Cachexia, paraplegia and survival. Body weight was monitored two or three times a week. Mice were killed when cachexia or paraplegia was observed. Mice were considered cachectic when lost over 20% of body weight from the maximum weight. Animals with loss of function in any limbs were considered paraplegic. Mice were killed by cervical dislocation under anesthesia. Maximum study length was 60 days but individual mice were sacrificed earlier when sacrifice criteria were met. Survival is reported as time from study day 0 to sacrifice day.

X-ray radiography. The development of osteolytic lesions was monitored in an anteroposterior position with the Faxitron Specimen Radiographic System MX-20 D12 (Faxitron Corporation, Wheeling, IL, USA) using the Faxitron Dicom 3.0 software. One radiograph (both hind limbs) per animal was taken on each X-ray occasion (31 kV, 10 seconds, 2x magnification). The number of lesions was counted and the lesion area were measured in hind limbs from the images with the MetaMorph image analysis software (Molecular Devices, LLC, Sunnyvale, CA, USA).

Fluorescence imaging of GFP. Tumor burden was monitored by imaging the fluorescence emitted by MDA-MB-231(SA)-GFP cells using an LT 9 GFP-imaging system LT-MACIMSYSPLUSC (Lightools Research, Encinitas, CA, USA). Excitation wavelength was 470 with 40 nm band-pass and emission wavelength cut-off filter was at 515 nm.

Figure 1.
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Figure 1.

The decay chain of 224Ra, including details on each nuclides' half-life, main mode of decay and mean energies.

Statistical analysis. The data were analyzed using a statistical software R (version 3.1.2, www.r-project.org) or SigmaPlot version 12.5 (Systat Software, Inc., Chicago, IL, USA). For the numeric end-point parameters, normality and homogeneity of group variances were checked. If these criteria were fulfilled as such or after some data transformation (logarithmic, square root, reciprocal), the data were analyzed using one-way ANOVA followed by Tukey's HSD test. If the criteria were not fulfilled, the results were analyzed using Kruskal-Wallis test followed by Mann-Whitney U-test. The weight curves were analyzed using a mixed model and model contrasts. The model had effects for treatment and their interaction as well as subject-wise random effect. Value at day -4 was used as baseline covariate in the models. Survival curves were estimated using the Kaplan-Meier estimator and compared using the log-rank test by pairwise comparisons. Paraplegia and cachexia analysis were carried out using Fisher's exact test by pairwise comparisons. The obtained p-values were adjusted for multiple comparisons. The p threshold for significance was 0.05.

Results

Radium-224-solution prolonged symptom-free survival in mice with breast cancer metastases. A single injection of 224Ra-solution extended significantly symptom-free survival in a dose-dependent manner (Figure 2A). The control groups receiving either the sodium chloride vehicle or EDTMP in sodium chloride solution had no survivors beyond day 26. While 45, 91 and 179 kBq/kg 224Ra-solution groups had 25% (3 of 12), 67% (8 of 12) and 92% (11 of 12) survivors at day 26, respectively (Figure 2A). Mean survival (time to sacrifice) was 22 days and 23 days in the vehicle and EDTMP groups, respectively (Figure 2B). The median survival of mice was around 14% (3 days), 27% (6 days) and 41% (9 days) longer in 45, 91 and 179 kBq/kg 224Ra groups compared to the sodium chloride vehicle group (Figure 2B). The increase in survival for the mice treated with 224Ra solution relative to the two control groups was significant for all 224Ra groups (Figure 2B).

Figure 2.
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Figure 2.

Effect of 224Ra-solution in prolonging symptom-free survival (A) and the day of sacrifice (B) of nude mice. Metastases were established in mice by injection of 105 MDA-MB-231(SA)-GFP breast cancer cells in the left cardiac ventricula. Animals were treated two days after cell inoculation. The treatment groups received 45, 91 or 179 kBq/kg of 224Ra-solution administered by tail vein injection, whereas animals in the control groups were injected with vehicle (sodium chloride) or bone-seeking EDTMP (25 mg/kg). (A) Symptom-free survival data were estimated by the Kaplan-Meier analysis followed by the log-rank test against the EDTMP group. (B) Bottom of the boxes represent the 25th percentile, dashed lines represent means, solid lines represent medians, top of the boxes represent the 75th percentile, and whiskers represent the 5th and 95th percentiles. Asterisk (*) indicates statistically different values when comparing the groups to the EDTMP group (***p<0.001, **p<0.01).

Figure 3.
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Figure 3.

Effect of 224Ra-solution on mouse body weight changes (A) and mouse body weight relative to baseline at the time of sacrifice (B). (B) Bottom of the boxes represent the 25th percentile, dashed lines represent means, solid lines represent medians, top of the boxes represent the 75th percentile, and whiskers represent the 5th and 95th percentiles. The data (B) were analyzed using the Kruskal-Wallis test.

The body weight of mice increased during the first two weeks and later started to decline in all groups (Figure 3A). These results showed that there was no significant difference in the body weight of mice between treatment and control groups at the beginning of the study, during the experiment and at sacrifice day (p>0.05, Figure 3).

Radium-224-solution prevented tumor-related paraplegia. Paraplegia occurred in 83% of the animals in the vehicle group and was the main cause for sacrifice in this group (Figure 4A). Radium-224-solution treatment decreased the incidence of paraplegia in a dose-dependent manner (Figure 4A). Paraplegia was not observed in 179 kBq/kg 224Ra-solution group (Figure 4A). The main cause for sacrifice of mice in this group was cachexia, which was significantly higher than in the control groups (Figure 4B).

Figure 4.
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Figure 4.

Percentage of mice with paraplegia (A) and cachexia (B) at the time of sacrifice. The data were analyzed using the Fisher's exact test.

Radium-224-solution inhibited bone metastasis in mice. Osteolytic lesions in the hind limbs of mice were detected and analyzed by X-ray radiography (Figure 5). Tumor growth in the whole body was detected and analyzed by fluorescence imaging of GFP (Figures 6 and 7). In the present study, all mice (100%) in all groups developed bone metastasis after inoculation of MDA-MB-231(SA)-GFP cells into the left ventricle of the heart, which is a well-established technique to develop bone metastases (17). There was no significant difference between the two control groups. (Figures 5, 6 and 7). After 224Ra-solution treatment the formation of osteolytic lesions was inhibited in a dose-dependent manner (Figure 5B). High statistical significance (p<0.001) was observed for highest activities of 224Ra (91 and 179 kBq/kg) when compared to control groups. Animals in 179 kBq/kg 224Ra-solution group had around five times smaller area of osteolytic lesions and a 1.5-fold less osteolytic lesions at sacrifice when compared to EDTMP control group (Figures 5B and C).

MDA-MB-231(SA)-GFP cells formed tumors in the bone sites such as in the scapula, wrist, ribs, spine, hind limbs and iliac bone (bright green spots in the images in Figure 6 indicated by white arrows as examples in the vehicle group images A and B). Total tumor burden at sacrifice was not decreased by 224Ra-solution treatment (Figure 7A) but it must be noted that the animals treated with 224Ra-solution had more time to develop soft tissue metastases as these animals lived longer than the controls. However, one difference in the location of tumors was observed. Less tumor burden was seen at bone sites in mice treated with 224Ra-solution, especially in the 179 kBq/kg 224Ra-solution group (Figure 6) but tumor burden was still noted in the head area and eyes (Figure 6, red arrows). The number of tumor foci at bone sites was decreased strongly by 224Ra-solution treatment by 2, 2.5 and 10 times, respectively (Figure 7B).

Discussion

The current work represents the first study of an injectable solution of the α-emitter 224Ra in a cancer model. We chose to add EDTMP to improve the properties of the 212Pb progeny. Previously, 224Ra without EDTMP was used in treatment of ankylosing spondylitis (6-8). Long-term toxicity and carcinogenicity data in humans exist from this usage (6, 19-24) indicating a quite acceptable safety profile compared with other cancer therapeutics, and could possibly be further improved upon by adding EDTMP for soft tissue protection and by using a more moderate dosing intensity. Our data demonstrate for the first time that a single treatment with 224Ra-solution resulted in a significant prolongation of symptom-free survival, reduction of paraplegia induction and decreased number and areas of bone metastases. Radium-224 decays with a physical half-life of 3.64 days via several progenies to 208Pb by emitting four α-particles within a relatively short time span (Figure 1). The antitumor effect is most likely linked to the delivery of an intense and highly localized radiation zone from α-particles targeting the bone surfaces. The treatment with 224Ra-solution seemed to be well tolerated because no signs of body weight loss could be seen in the groups of treated animals (Figure 3).

In the present study the tetraphosphonate chelator, EDTMP, with a high affinity for areas of active bone turnover (25), had no significant effect by itself on survival of mice, reduction of paraplegia and cachexia or formation of bone metastasis (Figures 2, 3, 4, 5, 6 and 7). Lead-212 (t1/2=10.6 h), which is the longest living progeny in the 224Ra series, yields indirectly one α-particle (via 212Bi). By adding EDTMP to the 224Ra solution, soft tissue exposure to 212Pb present in the 224Ra-solution was reduced (16).

Figure 5.
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Figure 5.

Representative radiographs (A) of hind limbs of mice bearing tumors after intracardiac inoculation of MDA-MB-231(SA)-GFP cells. Osteolytic bone metastases are indicated by arrows. The images were taken at day of sacrifice. Total osteolytic lesion area (B) and total number of osteolytic lesions (C) per mouse at the time of sacrifice. Development of bone metastases on hind limbs was monitored by X-ray radiography. The results are shown as the sum of areas of bone lesions (B) or the count of individual bone lesions (C) in right and left tibia and femur per animals. Bottom of the boxes (B) represent the 25th percentile, dashed lines represent means, solid lines represent medians, top of the boxes represent the 75th percentile, and whiskers represent the 5th and 95th percentiles. Prior to statistical analysis, the data (B) were transformed using logarithmic transform. Statistical analysis was performed using ANOVA (A) or Kruskal-Wallis test (C). As statistical differences were observed for b and c, the pairwise comparison was performed using Tukey's HSD test (B) or Mann-Whitney U-test (C). Asterisk (*) indicates statistically different values when comparing the animals in EDTMP group (***p<0.001, **p<0.01).

Figure 6.
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Figure 6.

Representative whole-body fluorescence images of GFP of mice bearing tumors after intracardiac inoculation of MDA-MB-231(SA)-GFP cells. (A) Ventral view, (B) dorsal view. The skeleton has been exposed and therefore, visceral organs, heart and lung have been removed. Example metastases are indicated by white (bone metastases) and red (eye metastases) arrows. These images were taken at the day of sacrifice.

Spinal cord compression from spinal metastasis is a common complication in breast and prostate cancers and, if left untreated, permanent paraplegia or quadriplegia will occur (26). Patients with paraplegia have significantly decreased quality of life and shortened survival. Therefore, it is important to find effective treatments for preventing or improving the outcome of metastatic spinal cord compression. The intracardially injected MDA-MB-231(SA) cells form osteolytic lesions and induce paraplegia of the hind legs, both of which are characteristic of osteolytic metastasis (17, 27). The treatment with 224Ra-solution (179 kBq/kg) prevented paraplegia totally (Figure 4A). This happened because 224Ra had a direct effect on the tumor foci in the spinal bones, strongly slowing down tumor growth and decreasing foci number (Figure 6).

In the study by Suominen et al. (4) 223Ra was given at a dosage of 300 kBq/kg in mice of similar age receiving the same number of cells injected intracardially as in our current study. In one of their cohorts the animals received treatment on day two. It should be noted that since 223Ra (t1/2=11.4 days) and the 223Ra series also generate four α-particles with similar total energy as for the 224Ra series, 300 kBq/kg dose of 223Ra would be approximately five times the amount of radium cations as the 189 kBq/kg 224Ra dosing due to the longer half-life. Despite this large difference in radium dosing, the two treatments had similar efficacy in this model with life prolongation of about 41% (224Ra, 179 kBq/kg) and 45% (223Ra, 300 kBq/kg), respectively, indicating that the 224Ra-solution had high biological activity.

Advantage of developing 224Ra based therapeutics lies in the well documented data on long term and short-term toxicity from its previous use in non-cancerous human subjects. From 1946 to 1975 in Germany repeated intravenous injections of up to 2 MBq of 224Ra per injection twice a week for months were given to patients with ankylosing spondylitis (7). Injected amounts were up to 140 MBq 224Ra (7). Such high cumulative doses of 224Ra led to increased incidences of myeloid leukemia and malignancies of bones, kidneys and thyroid, and 224Ra was abandoned since 1990 (6, 22). An elevated risk for malignant bone tumors, however, has not been proven after low-dose treatment with 10 MBq 224Ra (6). In Germany, 224Ra-Cl (224SpondylAT®) was approved for the intravenous administration in ankylosing spondylitis patients in 2000 (28). Patients now received the total activity of only 10 MBq, with 1 MBq (low-dose) per injection (28). The excess absolute risk associated with this treatment was estimated to be 0.2% for malignant bone tumors and 0.4% for leukemia (6). The 224Ra-Cl product was discontinued in 2005 (28). Since then, 224Ra has not been used in clinical settings. Moderate doses of 224Ra with complexed 212Pb may be an excellent option in cancer metastasis treatment as the complexion of 212Pb may reduce risk associated with soft tissue exposure to co-injected 212Pb.

The presence of disseminated MDA-MB-231(SA)-GFP cells in the bone marrow two days after inoculation of the cells was studied earlier in the same mouse model, and tumor cells were observed in all histological sections from control mice (4). Findings in the present study indicate the effectiveness of 224Ra-solution to reduce bone metastasis but not soft tissue metastases, which were noted in the eyes (Figure 6, visceral organs were removed). It is known that 224Ra has the highest uptake in the skeleton of animals and humans (6, 29). Uptake of cationic Ra into the bone is very fast with only 8% of the injected dose remaining in the circulating blood after 15 min. Eight hours after injection only 1.5% is still found in the blood (8). After injection, 224Ra is incorporated into areas of pathological ossification. Radium-224 is enriched in sites of active bone formation and exerts its analgetic effect by blocking the secretion of pro-inflammatory cytokines (8). Osteolytic demineralization shown in the current study is inhibited in addition to the anti-inflammatory and analgetic activity, emphasizing the clinical relevance of 224Ra for the treatment of bone metastases.

Figure 7.
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Figure 7.

Whole body (A) and bone sites (B) tumor burden at the time of sacrifice of mice. Development of metastases was monitored by fluorescence imaging of GFP. (A) The results are shown as the average of fluorescence area in two pictures (prone and supine positions) of each animal. (B) Assessed areas include spine and limbs referring to the reprehensive images in Figure 4B. Bottom of the boxes (A, B) represent the 25th percentile, dashed lines represent means, solid lines represent medians, top of the boxes represent the 75th percentile, and whiskers represent the 5th and 95th percentiles. Statistical analysis was performed Kruskal-Wallis test. No statistical differences were observed for whole body tumor burden (A). As statistical differences were observed for b, the pairwise comparison was performed using Mann-Whitney U-test (B). Asterisk (*) indicates statistically different values when comparing to the animals in the EDTMP group (***p<0.001, **p<0.01).

The used in vivo model of breast cancer bone metastasis MDA-MB-231(SA)-GFP developed not only bone metastases, but also soft tissue metastases (Figure 6) and that happens in patients too (30). As an alternative to EDTMP it is also possible to use a bifunctional chelating agent TCMC (1,4,7,10-tetra-(2-carbamoyl methyl)-cyclododecane) conjugated with a monoclonal antibody (for example, trastuzumab (31, 32)) that can be added to the 224Ra-solution in equilibrium with 212Pb to scavenge 212Pb (16, 18). Upon administration, the antibody moiety of 212Pb-TCMC-trastuzumab binds with high affinity to the extracellular domain of human epidermal growth factor receptor 2 (HER2) which is overexpressed on the surface of breast cancers (31, 32). After internalization 212Pb delivers a cytotoxic dose of α radiation (via 212Bi) to the HER2-expressing tumor cells. This could potentially affect the soft tissue component of metastatic or primary bone cancers as the antibody component could target tumor cells also in circulation and in soft tissues.

There are some limitations to this study. Further studies are needed to evaluate the effects of 224Ra-solution treatment on more established breast cancer bone metastases as opposed to the micrometastatic setting used in the current study. The level of activity in this study could probably have been significantly increased due to moderate toxicity of the bone seeking α emitter 223Ra in mice (33), and 224Ra, with its shorter half-life, is expected to be similar or less toxic per kBq compared with 223Ra. Additionally, recently reported potential prognostic targets for bone metastatic disease in breast cancer, such as circulating MIC1/GDF15 levels and follistatin expression (34-35), can be investigated before and after treatment with 224Ra-solution to correlate with treatment outcome.

Our findings show that a single dose of 224Ra-solution prolonged survival time and lowered the incidence of paralysis and bone metastases in nude mice with breast cancer micrometastases. Radium-224 is a promising candidate for the treatment of breast cancer patients with bone metastases.

Acknowledgements

The study was financially supported by Sciencons AS (Oslo, Norway).

Footnotes

  • This article is freely accessible online.

  • Received January 9, 2018.
  • Revision received February 27, 2018.
  • Accepted March 1, 2018.
  • Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved

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Anticancer Research: 38 (4)
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Vol. 38, Issue 4
April 2018
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Antitumor Activity of Novel Bone-seeking, α-emitting 224Ra-solution in a Breast Cancer Skeletal Metastases Model
ASTA JUZENIENE, JENNI BERNOULLI, MARI SUOMINEN, JUSSI HALLEEN, ROY HARTVIG LARSEN
Anticancer Research Apr 2018, 38 (4) 1947-1955;

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Antitumor Activity of Novel Bone-seeking, α-emitting 224Ra-solution in a Breast Cancer Skeletal Metastases Model
ASTA JUZENIENE, JENNI BERNOULLI, MARI SUOMINEN, JUSSI HALLEEN, ROY HARTVIG LARSEN
Anticancer Research Apr 2018, 38 (4) 1947-1955;
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Keywords

  • bone metastasis
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  • radium-224
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