Article Figures & Data
Figures
- Figure 1.
Cytotoxicity of methadone (A), cisplatin (B) and combination of both agents (C) against urinary bladder cancer cells as measured by WST-1 viability assay after 72 h. Mean values±SD of 3-4 independent experiments in triplicates are shown. Significantly different at *p≤0.05, **p≤0.01, ***p≤0.001, and ****p≤0.0001; ns, not significant.
- Figure 2.
A: Cytotoxicity of cisplatin (C), methadone (M), and the combination of both agents (C+M) on T24 cells as measured by WST-1 viability assay after 72 h. Pan-caspase inhibitor QVD-OPh hydrate (QVD) was used as an inhibitor of apoptosis. B: Phase-contrast images of T-24 cells treated with cisplatin (C), methadone (M) or in combination of both (C+M) with/without QVD after 72 h.
- Figure 3.
A: Analysis of apoptosis by flow cytometry with annexin V (FL1-H) and propidium iodide (FL3-H). The percentage of positive cells in each quadrant is shown. T24 cells were incubated with cisplatin (C), methadone (M) or with both agents in combination (C+M) for 72 h. The apoptosis inducer camptothecin (CPT) and the necrosis inducer Triton X-100 were used as positive controls. B: Western blot analysis of cleaved poly (ADP-ribose) polymerase (PARP) and caspase-3 (cas-3) in lysed cells treated with cisplatin (C), methadone (M) or with a combination of both (C+M) after 72 h. β-Actin was detected as a loading control.
