Figure 1. Different patterns of epithelial to mesenchymal transition in liver metastases of various origins. a: Non-hybrid phenotype cells, E-cadherin−/vimentin−, in the replacement growth pattern of colorectal liver metastases (CRCLM) E-cadherin/vimentin double immunostaining, ×1,000 magnification. b: Isolated cell with hybrid phenotype in desmoplastic type of CRCLM, E-cadherin/vimentin double immunostaining, ×400 magnification. c: Clusters of hybrid cells, desmoplastic type of CRCLM, E-cadherin/vimentin double immunostaining, ×400 magnification. d: Isolated hybrid phenotype cells in the pushing type of CRCLM, E-cadherin/vimentin double immunostaining, ×400 magnification. e: Isolated cell basal hybrid phenotype in the replacement type CRCLM, E-cadherin/vimentin double immunostaining, ×400 magnification. f: The amoeboid/mesenchymal morphology of hybrid phenotype cell in the replacement type of CRCLM, E-cadherin/vimentin double immunostaining, ×1,000 magnification. g: Isolated hybrid phenotype cell in the pushing type CRCLM, keratin 8,18/vimentin double immunostaining, ×1,000 magnification. h: Keratin 8,18 expression in metastatic epithelial phenotype cells and hepatocytes in the replacement type of a pancreatic liver metastasis keratin 8,18/vimentin double immunostaining, ×400. i: Keratin 8/18 immunostaining with intensity value of 3 in hepatocytes close to the portal spaces, keratin 8,18/vimentin double immunostaining, ×200 magnification.