Article Figures & Data
Figures
- Figure 1.
Effect of Mn on cell viability of PC3 cells (a), DU145 (b) and LNCaP (c) prostate cancer cells. Cells were treated with different concentrations of Mn for 24, 48, 72 and 96 h and the viability was analyzed by dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide. Data are expressed as mean±SEM of two independent experiments performed in triplicates. Letters indicate significant difference at: ap<0.05, bp<0.01, cp<0.001 and dp<0.0001 relative to the control (0).
- Figure 2.
Morphology of PC3 (a), DU145 (b) and LNCaP (c) prostate cancer cells exposed to different concentrations of Mn for 24, 48, 72 and 96 h. Ctrl: Control. Images magnified ×20 with digital zoom.
- Figure 3.
Cell-cycle analysis of cells in G0/G1 after Mn treatment in PC3 (a), DU145 (b) and LNCaP (c) presented as a percentage of that of the control cells (not exposed to Mn). Data are expressed as mean±SEM of two independent experiments. Statistically significantly different from the control (0 μM Mn) at: *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.
- Figure 4.
Analysis of apoptosis by flow cytometry after annexinV-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining. PC3 (a), DU145 (b) and LNCaP (c) cells were treated with different concentrations of Mn for 48 h. Annexin V indicates early apoptosis, AnnexinV/PI indicates late apoptosis and PI indicates necrotic cells. Data are expressed as mean ± SEM of two independent experiments. Statistically significantly different from the control (0 μM Mn) at: *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.
- Figure 5.
Manganese concentration (μM) in PC3, LNCaP and DU145 cells after exposure to different concentration of Mn for 48 h. Significantly different at ****p<0.0001.
- Figure 6.
Illustration of the relationship between cell viability (MTT%) and intracellular concentration of Mn (μM) after exposing PC3 (a), DU145 (b) and LNCaP (c) cells to different concentrations of Mn for 48 h. x-Axis shows the inverse logarithm of Mn concentration.
