Article Figures & Data
Figures
- Figure 1.
Effects of nonsteroidal anti-inflammatory drugs on blast colony formation BCF in methylcellulose. Full titration curves of NS-398 (NS) and indomethacin (Ind) for the seven cell lines were generated. BCF per well in control culture was 129±10, 100±5, 115±2, 68±14, 125±8, 70±9 and 192±20 in HL-60, U-937, U-266, Mo7e, K-562, Raji and Daudi cells, respectively. The data shown represent the mean±SD of five replicate cultures. The data were normalized as the percentage of the control value in each experiment. Control medium contained 0.38% dimethyl sulfoxide alone. *p<0.05 when compared to control cultures.
- Figure 2.
Effects of nonsteroidal anti-inflammatory drugs on cumulative clonogenic cell recovery in long-term suspension cultures of HL-60, U-937, U-266, Mo7e, K-562, Raji and Daudi cells. Cells were cultured in suspension with 0.38% dimethyl sulfoxide alone (Cont) or with dimethyl sulfoxide plus NS-398 (NS) or indomethacin (Ind). The data shown represent the mean±SD of five replicate cultures. Relative slope (rSLP) was expressed as inhibition relative to the control (1.0). *p<0.05 when compared to Cont.
- Figure 3.
Senescence and necrosis in liquid suspension cultures of HL-60, U-937, U-266, Mo7e, K-562, Raji and Daudi cells. Cells were treated with 0.38% dimethyl sulfoxide alone (Cont) or with dimethyl sulfoxide plus NS-398 (NS) or indomethacin (Ind). The percentage of cells stained with SA-β-galactosidase (senescent, Sen) or trypan blue (necrotic, Nec) was determined. The data shown represent the mean±SD of three separate measurements. *p<0.05 when compared to Cont.
- Figure 4.
The relative apoptotic cell fraction determined with flow cytometric analysis. HL-60, U-937, U-266, Mo7e, K-562, Raji and Daudi cells were treated with 0.38% dimethyl sulfoxide alone (Cont) or with dimethyl sulfoxide plus NS-398 (NS) or indomethacin (Ind). The apoptotic cell fraction was determined as described in the Materials and Methods Section. The data were normalized as the percentage of the control value in each experiment. The percentage of apoptotic cells in control cultures was 0.3%±0.7% (n=7), 4.8%±5.6% (n=7), 3.1%±2.6% (n=7) and 1.1%±0.6% (n=4) at day 0, 4, 7 and 10, respectively. *Enhancement and reduction of apoptosis was defined as ≥200% and <50%, respectively.
- Figure 5.
Effects of nonsteroidal anti-inflammatory drugs on telomerase activity in HL-60, U-937, U-266, Mo7e, K-562, Raji and Daudi cells. Cells were treated with 0.38% dimethyl sulfoxide alone (Cont) or with dimethyl sulfoxide plus NS-398-398 (NS) or indomethacin (Ind). The data shown represent the mean±SD of three separate measurements. *p<0.05 when compared to Cont.
