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Research ArticleExperimental Studies

Dynamic Phenotypic Transition of Breast Cancer Cells In Vitro Revealed by Self-floating Cell Culture

YUSUKE OKU, NAOYUKI NISHIYA, KAYOKO TSUDA, MASAHIKO SHIBAZAKI, CHIHAYA MAESAWA and YOSHIMASA UEHARA
Anticancer Research April 2017, 37 (4) 1793-1797;
YUSUKE OKU
1Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical University School of Pharmacy, Iwate, Japan
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  • For correspondence: yoku@iwate-med.ac.jp
NAOYUKI NISHIYA
1Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical University School of Pharmacy, Iwate, Japan
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KAYOKO TSUDA
1Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical University School of Pharmacy, Iwate, Japan
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MASAHIKO SHIBAZAKI
2Department of Tumor Biology, Institute of Biomedical Science, Iwate Medical University, Iwate, Japan
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CHIHAYA MAESAWA
2Department of Tumor Biology, Institute of Biomedical Science, Iwate Medical University, Iwate, Japan
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YOSHIMASA UEHARA
1Departments of Integrated Information for Pharmaceutical Sciences, Iwate Medical University School of Pharmacy, Iwate, Japan
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Abstract

Background: Breast tumor heterogeneity leads to phenotypic diversity, such as tumor-initiating and metastatic properties and drug sensitivity. Materials and Methods: We found that a self-floating cell (SFC) culture enriches a drug-resistant subpopulation in a HER2-positive breast cancer cell line. SFCs were analyzed for cancer stem cell markers, gene expression profiles, and sensitivity for anticancer drugs. Results: SFCs expressed cancer stem cell markers, such as aldehyde dehydrogenase (ALDH) activity and elevated HER2 autophosphorylation. Gene expression profiles of SFCs showed a dramatic difference compared to those of parental or forced floating cells. SFCs also expressed CD133, a marker of drug resistance, and resisted cytotoxic drugs by drug efflux transporters. In contrast, HER2 kinase inhibitors efficiently reduced SFC viability. Conclusion: SFCs enrich drug-resistant subpopulations even in vitro and might reflect the highly plastic nature of breast cancer cells even in vitro.

  • Tumor heterogeneity
  • breast cancer
  • HER2
  • drug resistance
  • Received January 27, 2017.
  • Revision received March 13, 2017.
  • Accepted March 14, 2017.
  • Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
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Anticancer Research: 37 (4)
Anticancer Research
Vol. 37, Issue 4
April 2017
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Dynamic Phenotypic Transition of Breast Cancer Cells In Vitro Revealed by Self-floating Cell Culture
YUSUKE OKU, NAOYUKI NISHIYA, KAYOKO TSUDA, MASAHIKO SHIBAZAKI, CHIHAYA MAESAWA, YOSHIMASA UEHARA
Anticancer Research Apr 2017, 37 (4) 1793-1797;

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Dynamic Phenotypic Transition of Breast Cancer Cells In Vitro Revealed by Self-floating Cell Culture
YUSUKE OKU, NAOYUKI NISHIYA, KAYOKO TSUDA, MASAHIKO SHIBAZAKI, CHIHAYA MAESAWA, YOSHIMASA UEHARA
Anticancer Research Apr 2017, 37 (4) 1793-1797;
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Keywords

  • Tumor heterogeneity
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