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Research ArticleExperimental Studies

Analysis of the miRNA Profiles of Melanoma Exosomes Derived Under Normoxic and Hypoxic Culture Conditions

MICHAL WOZNIAK, LUKASZ PECZEK, LILIANA CZERNEK and MARKUS DÜCHLER
Anticancer Research December 2017, 37 (12) 6779-6789;
MICHAL WOZNIAK
1Department of Molecular Biology of Cancer, Medical University of Lodz, Lodz, Poland
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LUKASZ PECZEK
2Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland
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LILIANA CZERNEK
2Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland
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MARKUS DÜCHLER
2Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland
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  • For correspondence: mduchler@cbmm.lodz.pl
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Abstract

Background/Aim: MicroRNAs (miRNAs) transported in melanoma-derived exosomes function as intercellular messengers supporting tumor survival and progression. Hypoxia increases melanoma phenotypic plasticity, drug resistance, and metastasis. Materials and Methods: We determined the miRNA profiles in exosomes derived from melanoma cells grown under hypoxic and normoxic conditions by microarray analyses and reverse transcription-polymerase chain reaction (RT-PCR) in order to analyze the potential influence of vesicle-transported miRNAs on cancer-related pathways and transcriptional programs. Results: Despite phenotypical differences of the four cell lines used, their exosomes shared the majority of miRNAs. The levels of three miRNAs were higher in normoxic exosomes, whereas 15 miRNAs were significantly more abundant under hypoxic conditions. Pathway analysis pointed at several cellular processes contributing to proliferation, drug resistance, and modification of the tumor microenvironment, including immunosuppression. Conclusion: The miRNA-expression profiles of exosomes from patient-derived melanoma cells are modified by oxygen concentration and reflect the phenotypic changes of melanoma cells under different growth conditions.

  • Melanoma
  • exosomes
  • miRNA
  • pathway analysis
  • Received August 25, 2017.
  • Revision received September 27, 2017.
  • Accepted October 3, 2017.
  • Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
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Anticancer Research: 37 (12)
Anticancer Research
Vol. 37, Issue 12
December 2017
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Analysis of the miRNA Profiles of Melanoma Exosomes Derived Under Normoxic and Hypoxic Culture Conditions
MICHAL WOZNIAK, LUKASZ PECZEK, LILIANA CZERNEK, MARKUS DÜCHLER
Anticancer Research Dec 2017, 37 (12) 6779-6789;

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Analysis of the miRNA Profiles of Melanoma Exosomes Derived Under Normoxic and Hypoxic Culture Conditions
MICHAL WOZNIAK, LUKASZ PECZEK, LILIANA CZERNEK, MARKUS DÜCHLER
Anticancer Research Dec 2017, 37 (12) 6779-6789;
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Keywords

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