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Research ArticleExperimental Studies

The Synthetic Cannabinoid WIN 55,212-2 Elicits Death in Human Cancer Cell Lines

LEONIE MÜLLER, ARLO RADTKE, JENNIFER DECKER, MICHAEL KOCH and GAZANFER BELGE
Anticancer Research November 2017, 37 (11) 6341-6345;
LEONIE MÜLLER
1Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany
2Department of Neuropharmacology – Brain Research Institute, University of Bremen, Bremen, Germany
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ARLO RADTKE
1Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany
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JENNIFER DECKER
1Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany
2Department of Neuropharmacology – Brain Research Institute, University of Bremen, Bremen, Germany
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MICHAEL KOCH
2Department of Neuropharmacology – Brain Research Institute, University of Bremen, Bremen, Germany
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GAZANFER BELGE
1Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany
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  • For correspondence: belge{at}uni-bremen.de
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    Figure 1.

    Percentage of dead cells after treatment of A549, HoTu-10 and IMR-5 cells with different doses of (R)-(+)-WIN 55,212-2 mesylate salt (WIN). Data are the mean±standard deviation (n=3).

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    Figure 2.

    Boxplots of the percentage of dead A549, HoTu-10 or IMR-5 cells after treatment with (R)-(+)-WIN 55,212-2 mesylate salt (WIN). All n=9, except n=10 for HoTu-10 cells at 20 μM concentration and n=11 for IMR-5 cells at 10 μM. Significant differences between subgroups at *p<0.05; **p<0.01; ***p<0.001.

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    Figure 3.

    Agarose gel electrophoresis of DNA from A549 (A) and HoTu-10 (B) cells treated with 20 μM (R)-(+)-WIN 55,212-2 mesylate salt (WIN) and the according controls. In both parts, 6 μg DNA from treated cells (lane 1), 1 μg DNA from treated cells (lane 2), 6 μg DNA from control cells (lane 3), 1 μg DNA from control cells (lane 4) and a molecular weight marker (lane 5) were used. Arrows indicate the ladder pattern. No DNA-ladder pattern was visible on treatment of I MR-5 cells with WIN (data not shown).

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November 2017
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The Synthetic Cannabinoid WIN 55,212-2 Elicits Death in Human Cancer Cell Lines
LEONIE MÜLLER, ARLO RADTKE, JENNIFER DECKER, MICHAEL KOCH, GAZANFER BELGE
Anticancer Research Nov 2017, 37 (11) 6341-6345;

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The Synthetic Cannabinoid WIN 55,212-2 Elicits Death in Human Cancer Cell Lines
LEONIE MÜLLER, ARLO RADTKE, JENNIFER DECKER, MICHAEL KOCH, GAZANFER BELGE
Anticancer Research Nov 2017, 37 (11) 6341-6345;
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