Article Figures & Data
Figures
- Figure 1.
Stereomicroscopic imaging analyses of hepatoma cell lines (HuH-7: (A); HepG2: (B)) in vitro. Imaging was performed under white light and excitation light (excitation: 440 nm; emission: 575–675 nm). Red fluorescence of protoporphyrin IX (PpIX) was observed in both hepatoma cell lines treated with 5-aminolevulinic acid (5-ALA) under excitation light.
- Figure 2.
Stereomicroscopic imaging analyses in the hepatoma mouse model. This model developed hepatomas that were microscopically visible within 4 weeks after injection of enhanced green fluorescent protein (EGFP)-expressing HuH-7 cells into the spleen. Red fluorescence was observed in tumors with EGFP fluorescence.
- Figure 3.
Red fluorescence was observed at the incision surface of resected livers, which was parallel with the location of the tumor. Imaging under white light and excitation light.
- Figure 4.
Spectral analysis of the tumor and the background. Solid line indicates the fluorescence spectra in the tumor and dotted line indicates the background fluorescence spectra of the liver. Fluorescence with a peak of ~635 nm was also observed in these tumors using a spectral analytical system, which corresponds to the spectra of protoporphyrin IX (PpIX).
