Article Figures & Data
Figures
- Figure 1.
Gene and protein expression of aryl hydrocarbon receptor (AHR) in leukemia cell lines. A: Relative expression of AHR mRNA in the four leukemia cell lines Jurkat, Kasumi-1, NB4, and K562 as measured by real-time polymerase chain reaction. N.D.: Not detected, **p<0.01. B: Immunocytochemical images of AHR protein (green) in NB4 cells. Nucleic acid is stained with TOTO-3 iodide (blue). Scale bar=20 μm.
- Figure 2.
Effect of StemRegenin 1 (SR1) on cell proliferation and death of NB4 cells. NB4 cells were cultured in the presence of three different concentrations of SR1 [0.375 μM, 0.75 μM and 1.5 μM containing 0.1% dimethyl sulfoxide (DMSO)] or 0.1% DMSO only (control) for up to 4 days. A: The number of viable cells was determined at days 0, 2 and 4. **p<0.01. B: The percentage of dead cells at day 2 and 4 of culture was determined. n.s.: Not significant, *p<0.05, **p<0.01. C: Images of the cells under the microscope at day 2 and 4 of culture. D: Images of May-Grünwald Giemsa staining at day 4 of culture. Scale bar=20 μm.
- Figure 3.
StemRegenin 1 (SR1) up-regulated cell proliferation- and apoptosis-related genes in the NB4 cell line. Relative expression of B-cell CLL/lymphoma 2 (BCL2), BCL2 associated X protein (BAX), myeloid cell leukemia 1 (MCL1), cyclin D1 (CCND1), CCND2 and v-myc avian myelocytomatosis viral oncogene homolog (MYC) mRNAs of NB4 cells cultured with 1.5 μM SR1 containing 0.1% DMSO, or 0.1% DMSO only (control) were measured by real-time polymerase chain reaction. A: Expression of BCL2, BAX, MCL1, CCND1, CCND2 and MYC at day 2. B: Expression of CCND1, CCND2 and MYC at day 4. n.s.: Not significant, *p<0.05, **p<0.01.
- Figure 4.
Effect of StemRegenin 1 (SR1) on gene-expression profiles of the NB4 cell line. A: Scatter plot of DNA microarray comparing the signals in control with those in NB4 cells treated with SR1 for 4 days. Significantly up- (red) and down- (blue) regulated genes are indicated. B: Gene functional annotation using the DAVID software. The up- (left) and down- (right) regulated genes functional annotations with the top 10 are shown in order of significant difference from those of the control.
