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Research ArticlePROCEEDINGS OF THE CHINA –UNITED KINGDOM CANCER (CUKC) CONFERENCE 2015 (Cardiff, Wales, UK)

Effect of YangZheng XiaoJi Extract, DME-25, on Endothelial Cells and their Response to Avastin

SIONED OWEN, YONG GAO, XIUYI ZHI, CONG WEI, YILING WU and WEN G. JIANG
Anticancer Research March 2016, 36 (3) 1181-1192;
SIONED OWEN
1Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K.
2Cardiff University-Capital Medical University Joint Centre for BioMedical Research and Cancer Institute, Capital Medical University, Beijing, P.R. China
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YONG GAO
3Yiling Medical Research Institute, ShijiaZhuang, HeBei Province, P.R. China
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XIUYI ZHI
2Cardiff University-Capital Medical University Joint Centre for BioMedical Research and Cancer Institute, Capital Medical University, Beijing, P.R. China
4Department of Thoracic Surgery, Beijing XuanWu Hospital, Capital Medical University, Beijing, P.R. China
5Beijing Lung Cancer Centre, Capital Medical University, Beijing, P.R. China
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CONG WEI
3Yiling Medical Research Institute, ShijiaZhuang, HeBei Province, P.R. China
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YILING WU
3Yiling Medical Research Institute, ShijiaZhuang, HeBei Province, P.R. China
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WEN G. JIANG
1Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff, U.K.
2Cardiff University-Capital Medical University Joint Centre for BioMedical Research and Cancer Institute, Capital Medical University, Beijing, P.R. China
5Beijing Lung Cancer Centre, Capital Medical University, Beijing, P.R. China
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  • For correspondence: jiangw@cardiff.ac.uk
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Abstract

Background: Angiogenesis is a cellular process that has been identified as a key target for therapy in solid cancer. However, over the course of anti-angiogenic therapies, cancer cells acquire resistance to these therapies after an initial period of success. DME-25 is an extract from Yang Zheng Xiao Ji, a traditional Chinese medicine that has been reported to benefit patients with cancer by alleviating chemotherapy-associated symptoms and possibly inhibiting key cancer cell traits. This study aimed to explore if DME-25 on its own and in combination with avastin affected endothelial cell behaviour in vitro in the presence of hypoxic lung cancer-conditioned medium (CM). Materials and Methods: Two lung cancer cell lines, A549 and SK-MES-1, were exposed to hypoxic conditions (O2 ≤1%) for 4 h, after which CM, and RNA were collected. Transcript expression of several influential angiogenic markers in lung cancer cells were assessed following hypoxic/normoxic conditions. Lung cancer CM was added in combination with avastin and DME-25, before or after vascular endothelial growth factor (VEGF) depletion, to endothelial cells (HECV) and cell migration and microtubule formation were assessed in vitro. Results: HECV cell migration was reduced in the presence of avastin, although less efficiently in the presence of lung cancer CM. A combination of DME-25 and avastin with lung cancer CM significantly reduced HECV cell migration irrespective of culture under hypoxia or normoxia. Depletion of VEGF from the CM reduced the inhibitory capacity of avastin, however, it appeared to have little impact on the anti-angiogenic effects of DME-25. Conclusion: DME-25 inhibits tubule formation irrespectively of the factors secreted by normoxic or hypoxic lung cancer cell CM depleted of VEGF.

  • Lung cancer
  • avastin
  • traditional Chinese medicine
  • DME-25
  • hypoxia
  • endothelial cells
  • Received January 4, 2016.
  • Revision received February 17, 2016.
  • Accepted February 18, 2016.
  • Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research
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March 2016
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Effect of YangZheng XiaoJi Extract, DME-25, on Endothelial Cells and their Response to Avastin
SIONED OWEN, YONG GAO, XIUYI ZHI, CONG WEI, YILING WU, WEN G. JIANG
Anticancer Research Mar 2016, 36 (3) 1181-1192;

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Effect of YangZheng XiaoJi Extract, DME-25, on Endothelial Cells and their Response to Avastin
SIONED OWEN, YONG GAO, XIUYI ZHI, CONG WEI, YILING WU, WEN G. JIANG
Anticancer Research Mar 2016, 36 (3) 1181-1192;
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Keywords

  • lung cancer
  • avastin
  • traditional Chinese medicine
  • DME-25
  • hypoxia
  • endothelial cells
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