Article Figures & Data
Figures
- Figure 1.
Differential expression of keratin in oral SCC monolayers vs. multicellular spheroids (MCS). SCC9, SCC9β6 (β6), SCC9CAFyn (CA) and SCC9β6KDFyn (KD) cells were grown for 48 h either as a monolayer or MCS under serum-free conditions and analyzed by western blotting using a pan-keratin Ab. Expression of keratin was increased in all cell lines when grown as an MCS.
- Figure 2.
Mutations to Fyn kinase modulate the expression of vimentin. The SCC9, SCC9β6 (β6), SCC9CAFyn (CA) and SCC9β6KDFyn (KD) cells were grown either as a monolayer or an MCS and then examined for the expression of vimentin. When grown as a monolayer trace amounts were detected in the SCC9 and the SCC9β6 cells and more substantial levels were detected in the SCC9CAFyn and SCC9β6KDFyn cells. However when grown as an MCS, vimentin was detected only in the SCC9β6KDFyn cell line.
- Figure 3.
Fibronectin expression is altered by multicellular spheroid formation. (A) SCC9, SCC9β6 (β6), SCC9CAFyn (CA) and SCC9β6KDFyn (KD) cells were grown as a monolayer for 48 h and evaluated for fibronectin. SCC9 and the SCC9β6 cells expressed more FN (rvu: 2.8, 2.5 respectively) compared to SCC9CAFyn and SCC9β6KDFyn cells (rvu: 0.8, 1.0 respectively). (B) When cells were grown as an MCS for 48 h and evaluated for fibronectin, the overall expression of FN was decreased with the exception of the SCC9β6KDFyn cell line which showed an rvu of 4.
- Figure 4.
Hypoxic environment modulates protein expression in SCC9β6KDFyn cells. SCC9β6KDFyn cells were grown as an MCS in the presence or absence of CoCl2 for 48 h at 37°C to induce a hypoxic environment. HIF-1α expression was (rvu: 1.0); whereas expression of N-cadherin and FN were decreased approximately 66% for both (rvu 1.0 and 1.2; respectively).
