Abstract
Background/Aim: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. Materials and Methods: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. Results: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. Conclusion: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found.
Footnotes
-
Conflicts of Interest
The Authors declare no conflicts of interest.
-
Funding
This research was funded by the internal resources of the departments involved (Breast Functional Unit, Obstetrics and Gynaecology Department and Pathology Department from Hospital del Mar; and IDIBELL from Catalan Institute of Oncology); sample processing at IDIBELL institute was partially funded by resources from a grant from Lilly Foundation (Premio de Investigación Biomédica Preclínica 2012 F. Xavier Bosch).
- Received October 13, 2014.
- Revision received November 4, 2014.
- Accepted November 7, 2014.
- Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved