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Research ArticleClinical Studies

Combined Treatment of Breast Cancer Cell Lines with Vitamin D and COX-2 Inhibitors

MARC THILL, KATHRIN REICHERT, ALENA WOESTE, STEPHAN POLACK, DOROTHEA FISCHER, FRIEDERIKE HOELLEN, ACHIM RODY, MICHAEL FRIEDRICH and FRANK KÖSTER
Anticancer Research February 2015, 35 (2) 1189-1195;
MARC THILL
1Department of Gynecology and Obstetrics, Agaplesion Markus Hospital, Frankfurt am Main, Germany
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KATHRIN REICHERT
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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ALENA WOESTE
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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STEPHAN POLACK
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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DOROTHEA FISCHER
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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FRIEDERIKE HOELLEN
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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ACHIM RODY
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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MICHAEL FRIEDRICH
3Department of Gynecology and Obstetrics, Helios Hospital Krefeld, Krefeld, Germany
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FRANK KÖSTER
2Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany
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  • For correspondence: frank.koester@uksh.de
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    Figure 1.

    The influence of calcitriol on the growth of breast cancer cell lines. Cell growth of MCF-7 and MDA-MB-231 cells was assessed by the MTT assay after 72 h of treatment with various concentrations of calcitriol. The results are shown as mean±SEM; statistical significance: ***p<0.001; (Co, control).

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    Figure 2.

    Combined treatment with calcitriol and celecoxib on MCF-7 (A) and MDA-MB-231 (B) cells. The proliferation is shown as the mean±SEM of cultures compared to the solvent control. Statistical significance: ***p<0.001. For the combined treatments, compared to single treatments, (A): a, b and c=p<0.001 and (B): a=p<0.01, b and c=p<0.001 (Co, control; Ca, calcitriol; and Ce, celecoxib).

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    Figure 3.

    COX-2 protein expression in MDA-MB-231 cells after treatment with calcitriol and celecoxib. Western blot using COX-2 antibody and β-actin as a loading control (Co, control; Ca,calcitriol; Ce, celecoxib).

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Anticancer Research: 35 (2)
Anticancer Research
Vol. 35, Issue 2
February 2015
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Combined Treatment of Breast Cancer Cell Lines with Vitamin D and COX-2 Inhibitors
MARC THILL, KATHRIN REICHERT, ALENA WOESTE, STEPHAN POLACK, DOROTHEA FISCHER, FRIEDERIKE HOELLEN, ACHIM RODY, MICHAEL FRIEDRICH, FRANK KÖSTER
Anticancer Research Feb 2015, 35 (2) 1189-1195;

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Combined Treatment of Breast Cancer Cell Lines with Vitamin D and COX-2 Inhibitors
MARC THILL, KATHRIN REICHERT, ALENA WOESTE, STEPHAN POLACK, DOROTHEA FISCHER, FRIEDERIKE HOELLEN, ACHIM RODY, MICHAEL FRIEDRICH, FRANK KÖSTER
Anticancer Research Feb 2015, 35 (2) 1189-1195;
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Keywords

  • vitamin D
  • Celecoxib
  • cyclooxygenase-2
  • Breast cancer
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