Article Figures & Data
Figures
- Figure 1.
Chemical structure of Cucurbitacin D.
- Figure 2.
Growth kinetics of NSCLC-N6 cells after CucD treatment with continuous drug exposure. Data are means±SD of three separate experiments.
- Figure 3.
Flow cytometric analysis of the DNA content of NSCLC-N6 cells cultured for 72 h in the absence (control) or presence of various concentrations of CucD. Cells were stained with propidum iodide as described in Materials and Methods.
- Figure 4.
RPA analysis of the A3 probe in NSCLC-N6 cells. A: NSCLC-N6 cells were cultured for 3 days under the following conditions: untreated cells (1) and 2.5±0.6 μg/ml CucD (2). Total RNA was extracted and RNase protection performed using one probe for A3 and another for beta 2 microglobulin mRNA. B: Quantification of results was performed with Easy Win 32 (HRL).
- Figure 5.
Results of RACE PCR electrophoresed in 2% agarose gel and stained with ethidium bromide. A: First round of RACE PCR. RACE PCR products were generated, as described in the Materials and Methods with P3 and oligo d(G)12 primer by a nested PCR on the P2-oligo d(G)12 amplification (lane 1). B: Second round of RACE PCR: first PCR with R2 and oligod(G) primer has generated non-specific bands (lane 1). Nested PCR (with R3 - oligo d(G)12 primers) generated a specific band of 1100pb (lane2) on this sample. MW lane shows the lambda phage DNA Eco RI-Hind III digest as molecular weight marker. The control lane shows amplification without the DNA sample.
- Figure 6.
Flow cytometric analysis of the DNA content of NSCLC-N6 cells cultured for 48 h in the absence (control) (A) or presence of CucD (3 μg/ml) and /or Cdk1 antisense oligonucleotide (10 μM) (C and D). Cells were stained with propidium iodide as described in Materials and Methods.
