miRNA Maturation. Methods and Protocols

miRNA Maturation. Methods and Protocols. Edited by C. Arenz. 2014, pp. 192, Eur 101.64, ISBN: 978-1-62703-702-0. Springer Science + Business Media, New York, NY, USA.

In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These include established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. This practical book seeks to widen the view on miRNA as biological mediator and potential drug target.