Article Figures & Data
Figures
- Figure 1.
Cytotoxic effects of 3-methyladenine (3-MA), bafilomycin-A1 (Baf-A1) and chloroquine (CQ) combined treatment with gts in NTUB1 and N/P cells. (A), (B) NTUB1 and N/P cells were co-treated with 3-MA (1 mM) and various concentrations of gts (0, 0.3, 0.6, 1.2 μM) for 48 h. (C), (D) Cells were co-treated with Baf-A1 (2 nM) and various concentrations of gts (0, 0.3, 0.6, 1.2 μM) for 48 h. (E),(F) Cells were co-treated with CQ (10 μM) and various concentrations of gts (0, 0.3, 0.6,1.2 μM) for 48 h. Cell viability was measured by the MTT assay and the results are presented as the calculated cell growth inhibitory ratio. Experiments were repeated three times.
- Figure 2.
The detection of apoptosis in NTUB1 and N/P cells exposed to gts and Baf-A1. Cells were treated with gts (0, 0.3, 0.6, 1.2 μM) and combine treatment with bafilomycin-A1 (2 nM) for 48 h. After treatment, the cells were harvested and stained with annexin V-FITC/PI and the percentage of the apoptotic cells was analyzed by flow cytometry.
- Figure 3.
The detection of autophagy in NTUB1 and N/P cells exposed to gts and Baf-A1. Acridine orange was used to stain acidic vesicular organelles (AVOs) in gts (0, 0.3, 0.6, 1.2 μM)-and Baf-A1 (2 nM)-treated NTUB 1(A) and N/P (B) cells for 48 h. The percentage of AVOs was measured by flow cytometry.
- Figure 4.
Effect of gts combined treatment with Baf-A1 on protein expressions in NTUB1 cells. A, Cells (5x105 cells/6-cm dish) were co-treated with Baf-A1 (2 nM) and various concentrations of gts (0, 0.3, 0.6, 1.2 μM) for 48 h. Following treatment, cells were harvested and lysed for detection of protein expression related to apoptosis by western blotting. B, Cells were examined for protein expression related to autophagy by western blot. β-Actin was used as a loading control.
- Figure 5.
Effect of gts combined treatment with Baf-A1 on protein expressions in N/P cells. A, Cells were co-treated with bafilomycin-A1 (2 nM) and various concentrations of gts (0, 0.3, 0.6, 1.2 μM) for 48 h. Following treatment, cells were harvested and lysed for detection of protein expressions related to apoptosis by western blot. B, Cells were examined for protein expressions related to autophagy by western blot. β-Actin was used as a loading control.
- Figure 6.
Effect of gts and Baf-A1 on degradation of Beclin-1.A, NTUB1 were treated with Baf-A1 (2 nM) and various concentrations of gts (0, 0.3, 0.6, 1.2 μM) and MG-132 (20 μM) for 48 h. Following treatment, cells were harvested and lysed for detection of beclin-1 expression by western blotting. B, N/P cells (3x105) treated with Baf-A1 (2 nM) and various concentrations of gts (0, 0.3, 0.6, 1.2 μM) and MG-132 (20 μM) for 48 h. The amount of protein expression was determined by immunoblot experiments against the indicated antibodies. Expression analysis of β-actin served as a loading control.
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