Article Figures & Data
Figures
- Figure 1.
Western blot of C1-HeLa and C7-HeLa stable cell lines demonstrating expression of claudin-1 and claudin-7 proteins respectively. Non-claudin transfected HeLa (Null-HeLa) displayed no expression of claudin-1 or claudin-7. Beta-actin is used as internal control to verify equal protein loading.
- Figure 2.
Graph showing quantification of invaded claudin-1, claudin-7-transfected and untransfected Hela cells at 560 nm following staining and extraction. Error bars show±standard deviation (2A). Brightfield image of invaded claudin-1 transfected (2B), claudin-7-transfected (2C), and untransfected HeLa (2D) (original magnification, ×40) (arrows indicate invasive cells).
- Figure 3.
Graphs show average resistance per cm2 in ohms for cultured monolayers of Claudin-1 transfected, Claudin-7 transfected and untransfected Hela cells. Error bars indicate±standard deviation.
- Figure 4.
Graph showing proliferation of C1-Hela, C7-Hela and Hela-Null cell lines using MTT assay at 24, 48 and 72 h time points. Error bars are ±SD.
- Figure 5.
Gap closure assay showing migration of cells over 48 h time period. Initial width of gap was measured at 0-h timepoint as being 840 μm, 870 μm and 850 μm for C1-HeLa, C7-HeLa and Null-HeLa respectively. Null-HeLa showed partial migration of cells into the gap after 24-h and complete gap closure at 48-h timepoint. C1-HeLa showed partial gap closure at 24 h and 48 h but incomplete gap closure over 48 h period. C7-HeLa showed a similar pattern to C1-HeLa with partial but incomplete gap closure after 48 h time period.
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