Effect of Withania somnifera Root Extract on Spontaneous Estrogen Receptor-negative Mammary Cancer in MMTV/Neu Mice

Hematoxylin and Eosin (H&E), Ki67, and PCNA staining of the mammary glands of control and WS-treated mice (magnification ×20). (A): H&E-stained sections of mammary carcinomas in WS-treated and untreated mice. Mammary carcinomas of treated mice showed decreased staining for Ki67(B), PCNA (C) and cytokine CCL2 (D). (E): Labeling indices, as determined by Ki67 and PCNA staining, and CCL2 were 42%, 38% and 50% less, respectively, in treated (Trtd) mice than in those of controls (Crtl).